All PSGL-1 / CD162 reagents are produced in house and quality controlled, including 18 PSGL-1 / CD162 Antibody, 24 PSGL-1 / CD162 Gene, 2 PSGL-1 / CD162 IPKit, 5 PSGL-1 / CD162 Lysate, 5 PSGL-1 / CD162 Protein, 2 PSGL-1 / CD162 qPCR. All PSGL-1 / CD162 reagents are ready to use.
Recombinant PSGL-1 / CD162 proteins are expressed by HEK293 Cells with fusion tags as C-human IgG1-Fc, C-cleavage, C-His, C-human IgG1-Fc & His, C-mouse IgG1-Fc.
PSGL-1 / CD162antibodies are validated with different applications, which are WB, ELISA, IP, FCM, IHC-P.
PSGL-1 / CD162cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each PSGL-1 / CD162 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.