PSG6 / PSG10 Protein (His Tag)

Pregnancy specific beta-1-glycoprotein 6 Protein Datasheet

PSG6 / PSG10 Protein Product Information

• Synonym: PSG6, CGM3, PSG10, PSG12, PSGGB
• Protein Construction: A DNA sequence encoding the human PSG6 ( Met 1- His 424) (NP_001027020) was expressed, with a C-terminal polyhistidine tag.
• Source: Human
• Expression Host: Baculovirus-Insect cells

PSG6 / PSG10 Protein QC Testing

• Purity: > 87 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 35
• Molecular Mass: The secreted recombinant human PSG6 consists of 400 amino acids and predicts a molecular mass of 45.2 KDa. The apparent molecular mass of the protein is approximately 58 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PSG6 / PSG10 protein

PSG6 / PSG10 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PSG6 / PSG10 Protein Description

PSG6 is a member of pregnancy-specific glycoproteins (PSGs) which are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy. PSGs comprise a subgroup of the carcinoembryonic antigen (CEA) family, which belongs to the immunoglobulin superfamily. PSGs play a critical role in pregnancy success. PSGs regulate T-cell function. The regulation of T-cell function during pregnancy is likely the result of significant hormonal changes and may well involve immunoregulatory proteins derived from the placenta. PSGs are the most abundant placentally derived glycoproteins in the maternal serum. PSG1, PSG6, PSG6N, and PSG11 induce dose-dependent secretion of anti-inflammatory cytokines by human monocytes. Human and murine PSGs exhibit cross-species activity. Low PSG levels in the maternal circulation are associated with threatened abortions, intrauterine retardation and fetal hypoxia. Also, PGSs may participate in maternal vasculature remodeling through influencing on secretion of proangiogenic agents such as transforming growth factor-beta-1 (TGF-β1) and vascular endothelial growth factor (VEGF) by different cell types involved in the development of placenta.

References

1. Teglund S. et al., 1995, Biochem Biophys Res Commun. 211 (2): 656-64.
2. Grimwood J. et al., 2004, Nature. 428 (6982): 529-35.
3. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.