Human PRTN3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13699-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PRTN3 cDNA Clone Datasheet

PRTN3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_002777.3
cDNA Size:	771
Gene Synonym:	MBN; MBT; NP4; P29; PR3; ACPA; AGP7; PR-3; CANCA; C-ANCA
Source:	Human

PRTN3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens proteinase 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 355 G/A resulting in the amino acid Val substitution by Ile.

PRTN3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRTN3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRTN3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCACC GGCCCCCCAG CCCTGCCCTG GCGTCCGTGC TGCTGGCCTT GCTGCTGAGC
61       GGTGCTGCCC GAGCTGCGGA GATCGTGGGC GGGCACGAGG CGCAGCCACA CTCCCGGCCC
121      TACATGGCCT CCCTGCAGAT GCGGGGGAAC CCGGGCAGCC ACTTCTGCGG AGGCACCTTG
181      ATCCACCCCA GCTTCGTGCT GACGGCCGCG CACTGCCTGC GGGACATACC CCAGCGCCTG
241      GTGAACGTGG TGCTCGGAGC CCACAACGTG CGGACGCAGG AGCCCACCCA GCAGCACTTC
301      TCGGTGGCTC AGGTGTTTCT GAACAACTAC GACGCGGAGA ACAAACTGAA CGACATTCTC
361      CTCATCCAGC TGAGCAGCCC AGCCAACCTC AGTGCCTCCG TCGCCACAGT CCAGCTGCCA
421      CAGCAGGACC AGCCAGTGCC CCACGGCACC CAGTGCCTGG CCATGGGCTG GGGCCGCGTG
481      GGTGCCCACG ACCCCCCAGC CCAGGTCCTG CAGGAGCTCA ATGTCACCGT GGTCACCTTC
541      TTCTGCCGGC CACATAACAT TTGCACTTTC GTCCCTCGCC GCAAGGCCGG CATCTGCTTC
601      GGAGACTCAG GTGGCCCCCT GATCTGTGAT GGCATCATCC AAGGAATAGA CTCCTTCGTG
661      ATCTGGGGAT GTGCCACCCG CCTTTTCCCT GACTTCTTCA CGCGGGTAGC CCTCTACGTG
721      GACTGGATCC GTTCCACGCT GCGCCGTGTG GAGGCCAAGG GCCGCCCCTG A

Quality Control:cDNA sequencing results is available upon request.