Mouse PRPF4B Gene cDNA Clone / ORF Clone

PRPF4B cDNA Clone Datasheet

PRPF4B cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013830.2
• cDNA Size: 3024
• Gene Synonym: Prp4; Prpk; Prp4k; cbp143; MGC5960; 2610037H07; Prpf4b
• Source: Mouse

PRPF4B cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus PRP4 pre-mRNA processing factor 4 homolog B (yeast) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PRPF4B cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRPF4B cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRPF4B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGCCA CGGAGCCCCC GTCCCTGCGG GAGCAAGCTG AGATGGATGA TGCTGATAAT
61       TCTGAAAAGA GTGTAAATGA AGAAAATGGA GAGGTATCAG AAGACCAATC TCAAAATAAA
121      CACAGTCGTC ACAAAAAAAA GAAGCATAAA CACAGAAGTA AACATAAGAA ACATAAACAT
181      TCCTCAGAAG AAGATAGGGA TAAAAAACAC AAACATAAGC ATAAGCATAA GAAGCACAAA
241      CGGAAAGAAG TTATCGAGGC TTCCGACAAA GAAGGCCTGT CCCCTGCAAA AAGAACTAAG
301      CTTGATGATT TAGCTTTGCT GGAAGACTTG GAAAAGCAGA GAGCCTTGAT TAAAGCTGAG
361      CTTGATAATG AGTTAATGGA GGGGAAGGTC CAGTCTGGAA TGGGGCTCAT TTTGCAAGGC
421      TATGAGTCTG GCTCCGAAGA AGAAGGAGAG ATTCACGAAA AGGCAAGAAA TGGGAACAGG
481      TCCAGTACCA GGTCTTCCAG TACTCGGGGA AAACTTGAAA TTACGGACAA CAAAAATAGT
541      GCAAAGAAAC GAAGTAAGAG CAGATCCAAA GAACGGACCA GGCACAGATC GGACAAAAGG
601      AAAAGCAAGG GGGCTGGGGA AATGCTGAGA GAGAAAGCAA ACAGGAGCAA GTCCAAGGAG
661      AGGAGAAAAT CCAAAAGCCC ATCCAAAAGA AGCAAGTCTC AAGACCAAGC TAGAAAGTCA
721      AAGTCCCCTC CACTCAGACG GCGGTCCCAA GAGAAAGTGG GGAAGGCCAG ATCTCCTGCT
781      GAGGAGAAGA TGAAGAGTGA AGAGAAAGGC AAAATAAAGG ATAGGAAAAA ATCACCAATT
841      GTAAATGAAA GGAGTCGAGA TAGATCTAAA AAATCCAAGT CCCCAGTTGA CTTAAGAGAT
901      AAATCCAAAG ACAGAAGGTC AAGGTCCAAA GAGCGGAAGT CAAAACGATC TGAAATTGAC
961      AAAGAAAAGA AGCCAATTAA ATCACCTTCA AAGGATGCTT CTTCTGGGAA GGAAAATAGG
1021     TCACCCAGTA GAAGACCTGG CCGTAGTCCT AAAAGAAGAA GTTTGTCTCC AAAACTGCGA
1081     GACAAGTCAA GAAGAAGTAG GTCTCCGCTT CTCAATGACA GAAGATCTAA GCAGAGCAAA
1141     TCGCCTTCTC GAACTCTGTC TCCAGGCAGA AGGGCCAAGA GCCGATCCTT AGAAAGAAAA
1201     CGGAGAGAAC CAGAGAGGAG ACGACTTTCT TCTCCGAGAA CAAGACCTCG AGATGATATC
1261     CTTGGTAGAT GTGAACGGTC AAAAGATGCC AGCCCCATCA ATAGGTGGTC TCCAACCCGA
1321     AGAAGAAGTA GGTCTCCAAT TAGAAGGAGG TCCCGTTCCC CATTGAGACG CAGCAGGTCT
1381     CCTAGAAGAA GAAGCAGGTC TCCTCGGAGA AGAGATAGGA GCCGAAGGAG CAGGTCACGC
1441     TTGAGGAGAA GGTCTCGATC ACGGGGTGGT CATAGGAGAA GGAGCAGAAG CAAAGTAAAG
1501     GAAGATAAAT TTAAAGGAAG TCTCTCAGAA GGAATGAAAG TTGAGCAAGA GTCTTCATCT
1561     GATGATAACC TTGAAGATTT TGATGTGGAG GAGGAAGATG AAGAGGCTCT AATAGAACAG
1621     AGAAGAATAC AGAGGCAAGC AATTGTGCAG AAATATAAAT ATCTTGCTGA AGATAGCAAT
1681     ATATCTGTGC CATCTGAGCC AAGCAGCCCC CAGAGCAGCA CAAGATCACG ATCTCCTTCG
1741     CCAGACGACA TCTTGGAGCG AGTTGCTGCT GATGTAAAAG AATATGAGCG GGAGAATGTT
1801     GACACCTTTG AGGCCTCAGT AAAAGCCAAG CATAATCTAA TGACAGTTGA ACAGAATAAT
1861     GGCTCATCTC AGAAGAAGAT TTTGGCACCT GACATGTTTA CAGAATCTGA TGACATGTTT
1921     GCTGCTTATT TTGATAGTGC TCGTCTCCGA GCTGCTGGCA TTGGGAAAGA TTTCAAAGAA
1981     AATCCCAACC TCAGAGATAA TTGGACAGAT GCAGAAGGCT ATTACCGTGT GAACATCGGG
2041     GAAGTCCTAG ATAAACGTTA TAATGTGTAT GGCTACACTG GGCAGGGTGT GTTCAGCAAT
2101     GTTGTGCGTG CCAGGGATAA TGCGAGAGCC AACCAGGAAG TGGCTGTAAA GATCATCAGG
2161     AACAATGAAC TCATGCAAAA GACTGGTTTA AAAGAGCTAG AATTCTTGAA AAAACTTAAT
2221     GATGCTGATC CTGATGACAA GTTCCATTGC CTGCGCCTCT TCAGACACTT TTACCACAAA
2281     CAGCACCTCT GTCTCGTCTT TGAACCTCTC AGTATGAATT TGCGAGAGGT ATTAAAAAAA
2341     TATGGTAAGG ATGTCGGTCT CCATATTAAG GCTGTACGAT CGTACAGTCA GCAGTTGTTC
2401     CTGGCATTGA AACTCCTTAA AAGATGTAAT ATCTTACATG CAGATATCAA ACCGGATAAT
2461     ATTCTGGTTA ATGAATCCAA AACAATTTTA AAACTTTGTG ATTTTGGATC GGCCTCACAT
2521     GTTGCAGATA ATGACATTAC GCCTTATCTT GTCAGTAGAT TTTATCGTGC TCCGGAGATC
2581     ATTATAGGTA AAAGCTATGA CTACGGTATC GATATGTGGT CTGTAGGTTG TACCTTATAT
2641     GAACTCTACA CTGGAAAAAT CTTATTCCCT GGCAAAACTA ATAACCATAT GTTGAAGCTC
2701     GCCATGGACC TTAAAGGAAA AATGCCAAAT AAGATGATCC GGAAAGGTGT ATTCAAAGAT
2761     CAACATTTTG ACCAAAATCT GAACTTCATG TACATAGAGG TTGATAAAGT GACAGAGAGG
2821     GAGAAAGTTA CTGTCATGAG CACCATTAAT CCAACTAAGG ACCTGTTGGC TGACTTGATT
2881     GGGTGCCAGC GACTTCCTGA AGATCAACGT AAAAAAGTAC ACCAGCTAAA GGACTTGTTG
2941     GACCAGATCC TAATGTTGGA CCCAGCTAAG CGAATTAGCA TCAACCAGGC CCTGCAGCAT
3001     GCATTCATCC AGGAAAAAAT TTAA

 

Quality Control:cDNA sequencing results is available upon request.