Rat PROM1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80324-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PROM1 cDNA Clone Datasheet

PROM1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001110137.1
cDNA Size:	2481
Gene Synonym:	Prom; CD133; MGC156650; Prom1
Source:	Rat

PROM1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus prominin 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PROM1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PROM1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PROM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCTCG TATTCAGTGT CCTGCTGCTA CTGGGGCTGT GTGGAAAGAT GGCTTCAGGA
61       GGTCAGCCTG CATTCGATAA CACTCCCGGG GCTTTGAATT ATGAATTGCC TACCACAGAA
121      TATGAGACCC AAGACACCTT CAATGCTGGG ATTATTGACC CTCTCTACCA AATGGTGCAC
181      ATCTTCCTCA ACGTGGTCCA GCCGAATGAC TTCCCTCAAG ATTTGGTCAA AAAACTCATA
241      CAGAAGAGGT TTGACATCTC GGTTGATACC AAGGAGGTCG CCATCTATGA AATTGGAGTT
301      CTTATCTGCG TCATCCTGGG CCTGCTGTTC ATTTTCCTCA TGCCCCTGGT GGGCTTCTTC
361      TTTTGTATGT GCCGTTGCTG TAACAAATGC GGCGGAGAAA TGCACCAGCG GCAGAAGCAG
421      AACGAGTCGT GCCGGAGGAA GTGCTTAGCC ATCTCCCTCT TGCTGATTTG TCTGCTCATG
481      AGTCTTGGCA TCGCGTTTGG CTTTGTGGCC AACCAGCAGA CAAGGACTCG GATCCAAAGG
541      ACCCAGAAGC TGGCAGAGAG CAATTACAGA GACTTGCGGG CTCTCCTGAC TGAAGCCCCA
601      AAGCAAATCG ACTATATTCT GGGGCAATAC AACACAACCA AGAACAAGGC ATTCTCAGAC
661      CTGGACAGTA TCGATTCCGT GCTGGGAGGC AGAATAAAGG GCCAACTAAA ACCCAAAGTT
721      ACTCCTGTTC TCGAAGAGAT TAAGGCCATG GCAACAGCCA TTAGACAGAC CAAGGATGCC
781      CTGCAGAACA TGAGCAGCAG TCTGAAAAGT CTACGTGACG CAAGCACCCA GCTCAGTACC
841      AACCTGACGT CTGTGAGAAA CAGCATCGAG AATTCACTCA ACAGCAACGA CTGTGCTTCA
901      GACCCAGCCA GCAAGATCTG TGATAGCCTC AGACCACAGC TGAGCAATCT GGGGAGCAAC
961      CACAACGGAA GTCAGCTCCC ATCAGTGGAC AGGGAACTCA ACACCGTTAA TGATGTCGAC
1021     AGAACCGATC TGGAGAGCCT CGTCAAAAGG GGGTATATGT CGATTGATGA AATACCCAAT
1081     ATGATACAAA ACCAAACTGG GGATGTCATC AAAGATGTCA AAAAGACCTT GGATTCTGTT
1141     AGCTCCAAGG TTAAGAACAT GAGCCAAAGT ATTCCTGTCG AGGAGGTGCT GCTTCAGTTC
1201     TCCCACTACC TTAATGACAG CAACAGGTAC ATCCACGAGT CGCTGCCCAG AGTGGAAGAA
1261     TATGACTCAT ACTGGTGGCT GGGTGGCTTA ATAGTCTGCT TCCTGCTGAC CCTCATTGTG
1321     ACTTTCTTTT ACCTGGGCTT GCTGTGTGGT GTGTTTGGCT ATGACAAGCG TGCCACCCCA
1381     ACCAGAAGAG GCTGCGTGTC CAACACTGGA GGCATCTTCC TCATGGCTGG GGTTGGATTC
1441     AGCTTCCTTT TTTGCTGGAT ATTGATGATC CTTGTGGTTC TTACTTTTGT TGTTGGTGCA
1501     AATGTGGAAA AGTTGCTCTG TGAACCTTAT GAAAACAAGA AATTACTACA GGTTTTGGAT
1561     ACTCCCTACC TGCTCAACGA TCAATGGCAA TTCTATCTCT CTGGCATACT ACTCAAAAAC
1621     CCAGACATTA ACATGACCTT TGAGCAAGTC TACAGGGATT GCAAAAGAGG TCGAGGAGTG
1681     TATGCCACTT TTCAGCTTGA GAATGTCTTC AACATCACTG AGAATTTCAA CATTGAGAGG
1741     CTTTCTGAAG ACATAGTGAA GGAGTTGGAA AAGCTGAATG TGAACATTGA CAGCATCGAA
1801     CTGTTGGACA AAACAGGAAG GAAGAGCCTC GAGGACTTTG CACAATCTGG GATAGATAGA
1861     ATCAATTATT CCATGTACTT ACAGGAGGCT GAGAAACCCC CTACCAAAGT GGACCTGCTG
1921     ACATTTGCCT CTTTTCTGGA AACAGAAGCA AACCAGTTGC CTGACGGAAA TCTGAAACAG
1981     GCCTTCTTAA TGGATGCACA GAACATTCGA GCCATCCACC AGCAGCACGT CCCTCCTGTG
2041     CAGCAGTCAC TGAATTCATT AAAACAAAGT GTCTGGGCCC TTAAGCAAAC AAGCAGCAAG
2101     TTGCCGGAGG AAGTGAAGAA GGTCCTTGCC TCTTTGGATT CTGCTCAGCA TTTCCTCACC
2161     AGTAACCTCT CCTCCATCGT TATTGGAGAA ACAAAGAAGT TTGGGAGAAC AATAATAGGC
2221     TACTTTGAAC ATTATCTGCA ATGGGTCTTG TACGCCATCA CAGAGAAGAT GACATCCTGC
2281     AAACCCATGA TTACAGCAAT GGACTCTGCT GTTAATGGCA TTCTGTGTAG CTATGTTGCT
2341     GACCCTCTGA ATCTGTTCTG GTTCGGCATA GGGAAAGCCA CCATGCTCTT ACTTCCGGCT
2401     GTAATCATCG CTATCAAGTT GGCCAAGTAC TATCGCAGGA TGGATTCAGA GGATGTATAT
2461     GATGACCCGT CTCGATACTG A

Quality Control:cDNA sequencing results is available upon request.