Mouse PROCR Gene cDNA Clone / ORF Clone

PROCR cDNA Clone Datasheet

PROCR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011171.2
• cDNA Size: 729
• Gene Synonym: Ccca; Epcr; Ccd41; AI325044; Procr
• Source: Mouse

PROCR cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protein C receptor, endothelial DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 112 A/C resulting in the amino acid Asn substitution by His.

PROCR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PROCR cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PROCR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGACGA AGTTTCTGCC GCTACTGCTG CTGCTGCTGC CTGGCTGCGC CCTTTGTAAC
61       TCCGATGGCT CCCAAAGCCT GCATATGCTC CAGATCTCCT ATTTCCAAGA CCACCATCAT
121      GTGAGGCATC AGGGCAACGC CTCTCTGGGA AAACTCCTGA CACACACGCT GGAAGGCCCC
181      AGCCAAAACG TCACCATCCT CCAGCTACAG CCCTGGCAGG ATCCGGAGAG CTGGGAGCGC
241      ACAGAGAGTG GCCTGCAGAT CTATCTGACC CAGTTCGAAA GCCTGGTGAA GCTGGTATAT
301      CGCGAGCGCA AGGAGAACGT GTTCTTTCCT CTCACTGTTA GCTGCTCCCT GGGCTGCGAG
361      CTGCCGGAGG AGGAGGAGGA GGGCTCTGAA CCCCATGTCT TCTTCGATGT GGCCGTGAAT
421      GGAAGCGCTT TTGTAAGTTT CCGGCCAAAG ACTGCCGTGT GGGTGTCAGG CTCCCAGGAG
481      CCCTCCAAAG CAGCCAACTT CACCCTGAAG CAGCTCAATG CCTACAACCG GACTCGGTAT
541      GAACTGCAGG AATTTCTTCA GGACACTTGT GTGGAGTTCC TGGAGAACCA CATCACCACG
601      CAAAACATGA AAGGGAGCCA AACAGGTCGC TCTTACACAT CACTGGTCCT GGGCATCCTG
661      ATGGGCTGTT TCATAATCGC TGGTGTGGCC GTGGGCATCT TCATGTGCAC AAGTGGACGG
721      CGTTGCTAA

 

Quality Control:cDNA sequencing results is available upon request.