PROCR / EPCR Protein

Protein C receptor, endothelial Protein Datasheet

PROCR / EPCR Protein Product Information

• Synonym: PROCR, EPCR
• Protein Construction: A DNA sequence encoding the human PROCR (AAH14451.1) (Met1-Thr209) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

PROCR / EPCR Protein QC Testing

• Purity: (20.4+75.1) % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 18
• Molecular Mass: The recombinant human PROCR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 433 amino acids and has a predicted molecular mass of 48.9 kDa. The apparent molecular mass of the protein is approximately 120 and 65 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PROCR / EPCR protein

PROCR / EPCR Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PROCR / EPCR Protein Description

Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) or PROCR, is a receptor for Protein C. Protein C plays an important role in many metabolism processes in humans and other animals after activated by binding to Endothelial protein C receptor (EPCR). Because of the EPCR is found primarily on endothelial cells(cells on the inside of blood vessels), activated protein C is found maily near endothelial cells. Protein C is pleiotropic, with two main functions: anticoagulation and cytoprotection. Which function will be performed depend on whether or not protein C remains bind to EPCR after activated. The anticoagulation occur when it does not. When still bound to EPCR, activated protein C performs its cytoprotective effects.

References

1. Nicolaes GA. et al., 2003, Semin Vasc Med. 3 (1): 33-46.
2. Esmon CT. 2003, Chest. 124 (3): 26-32.
3. Mosnier LO. et al., 2007, Blood. 109: 3161-3172.