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Rat PRNP Gene cDNA Clone (full-length ORF Clone)
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Rat PRNP Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80379-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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PRNP cDNA Clone Datasheet

 

PRNP cDNA Clone Product Information

Gene Bank Ref.ID: NM_012631.2

cDNA Size:

768

Gene Synonym:

PrP; Prn; Prnp

Source: Rat

PRNP cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus prion protein DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PRNP cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRNP cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRNP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGAACC TTGGCTACTG GCTGCTGGCC CTCTTTGTGA CTACATGTAC TGATGTTGGC
61       CTCTGCAAAA AGCGGCCAAA GCCTGGAGGG TGGAACACTG GTGGAAGCCG GTACCCTGGG
121      CAGGGAAGCC CTGGAGGCAA CCGTTACCCA CCTCAGAGTG GTGGTACCTG GGGGCAGCCC
181      CATGGTGGTG GCTGGGGACA ACCTCATGGT GGTGGCTGGG GACAACCTCA TGGTGGTGGC
241      TGGGGTCAGC CCCATGGCGG GGGCTGGAGT CAAGGAGGGG GTACCCATAA TCAGTGGAAC
301      AAGCCCAGCA AGCCAAAAAC CAACCTCAAG CATGTGGCAG GGGCTGCCGC AGCTGGGGCA
361      GTAGTGGGGG GCCTTGGTGG CTACATGTTG GGGAGCGCCA TGAGCAGGCC CATGATCCAT
421      TTTGGCAACG ACTGGGAGGA CCGCTACTAC CGTGAAAACA TGTACCGCTA CCCTAACCAA
481      GTGTACTACA GGCCAGTGGA TCAGTACAGC AACCAGAACA ACTTCGTGCA CGACTGCGTC
541      AATATCACCA TCAAGCAGCA CACGGTCACC ACCACCACCA AGGGGGAGAA CTTCACCGAG
601      ACCGATGTGA AGATGATGGA GCGCGTGGTG GAGCAGATGT GCGTCACCCA GTACCAGAAG
661      GAGTCCCAGG CCTATTACGA CGGGAGAAGA TCCAGCAGCA CCGTGCTTTT CTCCTCCCCT
721      CCTGTCATCC TCCTCATCTC CTTCCTCATC TTCCTGATCG TGGGATGA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"