Mouse PRNP Gene cDNA Clone / ORF Clone

PRNP cDNA Clone Datasheet

PRNP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011170.2
• cDNA Size: 765
• Gene Synonym: PrP; PrPC; Sinc; CD230; PrPSc; Prn-i; Prn-p; PrP<C>; AA960666; AI325101; Prnp
• Source: Mouse

PRNP cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus prion protein DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PRNP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRNP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRNP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGAACC TTGGCTACTG GCTGCTGGCC CTCTTTGTGA CTATGTGGAC TGATGTCGGC
61       CTCTGCAAAA AGCGGCCAAA GCCTGGAGGG TGGAACACCG GTGGAAGCCG GTATCCCGGG
121      CAGGGAAGCC CTGGAGGCAA CCGTTACCCA CCTCAGGGTG GCACCTGGGG GCAGCCCCAC
181      GGTGGTGGCT GGGGACAACC CCATGGGGGC AGCTGGGGAC AACCTCATGG TGGTAGTTGG
241      GGTCAGCCCC ATGGCGGTGG ATGGGGCCAA GGAGGGGGTA CCCATAATCA GTGGAACAAG
301      CCCAGCAAAC CAAAAACCAA CCTCAAGCAT GTGGCAGGGG CTGCGGCAGC TGGGGCAGTA
361      GTGGGGGGCC TTGGTGGCTA CATGCTGGGG AGCGCCATGA GCAGGCCCAT GATCCATTTT
421      GGCAACGACT GGGAGGACCG CTACTACCGT GAAAACATGT ACCGCTACCC TAACCAAGTG
481      TACTACAGGC CAGTGGATCA GTACAGCAAC CAGAACAACT TCGTGCACGA CTGCGTCAAT
541      ATCACCATCA AGCAGCACAC GGTCACCACC ACCACCAAGG GGGAGAACTT CACCGAGACC
601      GATGTGAAGA TGATGGAGCG CGTGGTGGAG CAGATGTGCG TCACCCAGTA CCAGAAGGAG
661      TCCCAGGCCT ATTACGACGG GAGAAGATCC AGCAGCACCG TGCTTTTCTC CTCCCCTCCT
721      GTCATCCTCC TCATCTCCTT CCTCATCTTC CTGATCGTGG GATGA

 

Quality Control:cDNA sequencing results is available upon request.