Mouse PRMT5 Gene cDNA Clone / ORF Clone

PRMT5 cDNA Clone Datasheet

PRMT5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013768.3
• cDNA Size: 1914
• Gene Synonym: Jbp1; Skb1; Prmt5
• Source: Mouse

PRMT5 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protein arginine N-methyltransferase 5 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for eleven point mutations: 89 T/G resulting in the amino acid Leu substitution by Arg, 506 A/C resulting in the amino acid Glu substitution by Gly,331 T/C, 342 G/A, 477 C/A, 552 A/G, 1293 C/T, 1446 T/C, 1612 T/C, 1638 A/G, 1725 G/A not causing the amino acid variation.

PRMT5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRMT5 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRMT5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCGA TGGCAGTCGG AGGTGCTGGT GGCAGCCGCG TGTCCAGCGG GAGGGACCTG
61       AATTGCGTCC CCGAAATAGC TGACACACGG GGTGCTGTGG CCAAGCAGGG GTTTGATTTC
121      CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG
181      AATCGGCCTG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG
241      CTAATTGTGG GAAAGCTTTC TCCATGGATT CATCCAGACT CAAAAGTGGA GAAGATCCGA
301      AGGAACTCTG AAGCGGCTAT GTTACAGGAG CTGAATTTTG GAGCATATCT GGGTCTTCCA
361      GCTTTCCTAT TGCCCCTTAA TCAGGAAGAT AACACGAATC TGGCCAGAGT TCTGACCAAC
421      CACATCCACA CTGGCCACCA CTCTTCCATG TTCTGGATGA GGGTACCCTT GGTGGCACCA
481      GAGGACCTGA GAGATGATGT AATTGGGAAT GCCCCGACTA CACACACAGA GGAGTACAGT
541      GGAGAAGAGA AGACATGGAT GTGGTGGCAT AACTTTCGGA CTCTGTGTGA CTATAGCAAG
601      AGAATTGCAG TAGCTCTTGA AATTGGAGCT GACCTCCCGT CTAATCACGT CATTGACCGC
661      TGGCTTGGAG AGCCCATCAA AGCAGCCATT CTCCCCACCA GCATTTTCCT AACCAACAAG
721      AAAGGATTTC CTGTTCTTTC TAAGGTGCAG CAGAGGCTGA TCTTCCGGCT CCTCAAGTTG
781      GAAGTGCAGT TTATCATCAC GGGAACCAAC CACCACTCAG AGAAAGAGTT CTGTTCCTAC
841      CTCCAGTACT TGGAATACTT AAGCCAAAAT CGCCCTCCAC CCAATGCCTA TGAGCTCTTT
901      GCCAAAGGCT ATGAAGACTA TCTGCAGTCC CCACTCCAGC CTCTGATGGA CAATCTGGAA
961      TCTCAGACAT ATGAAGTGTT TGAAAAGGAC CCCATCAAAT ACTCTCAATA TCAGCAGGCT
1021     ATTTATAAAT GTTTGCTAGA CCGAGTACCA GAAGAAGAAA AGGAGACCAA TGTCCAGGTA
1081     CTTATGGTGC TGGGTGCAGG CCGGGGTCCT CTTGTGAATG CGTCTCTTCG GGCAGCCAAA
1141     CAGGCCGAGC GGCGGATCAG GCTGTATGCT GTGGAGAAGA ACCCCAATGC TGTGGTGACG
1201     CTAGAGAACT GGCAGTTTGA AGAATGGGGG AGCCAGGTGA CAGTTGTCTC ATCAGACATG
1261     CGGGAATGGG TGGCTCCGGA GAAAGCTGAC ATTATTGTCA GTGAGCTTCT GGGTTCCTTT
1321     GCCGACAACG AGCTGTCACC TGAGTGTCTG GATGGAGCAC AGCACTTCCT GAAAGATGAT
1381     GGCGTGAGCA TCCCTGGAGA ATACACCTCC TTCCTGGCTC CCATTTCTTC CTCTAAGCTG
1441     TACAACGAGG TCCGTGCCTG TCGGGAAAAG GACCGCGATC CTGAGGCACA GTTTGAGATG
1501     CCTTATGTGG TTCGGTTGCA CAACTTCCAC CAGCTCTCTG CTCCTAAGCC CTGCTTTACC
1561     TTCAGCCATC CCAACCGAGA TCCTATGATT GACAACAACC GCTACTGTAC CCTGGAGTTT
1621     CCTGTGGAGG TGAACACGGT GCTTCATGGC TTCGCAGGCT ACTTTGAGAC TGTGCTTTAC
1681     CGGGACATCA CTCTGAGTAT CCGCCCAGAG ACTCACTCTC CTGGAATGTT CTCATGGTTC
1741     CCCATCTTCT TCCCCATTAA GCAGCCCATC ACGGTGCACG AAGGCCAGAA CATCTGTGTG
1801     CGTTTCTGGC GATGCAGCAA TTCCAAGAAA GTGTGGTACG AGTGGGCGGT GACGGCCCCC
1861     GTCTGTTCTT CTATTCACAA CCCTACCGGC CGCTCCTATA CCATTGGCCT CTAG

 

Quality Control:cDNA sequencing results is available upon request.