PRMT5 / SKB1 Antibody

PRMT5 / SKB1 Antibody Datasheet

PRMT5 / SKB1 Antibody Product Information

• Immunogen: Recombinant Human PRMT5 / SKB1 protein (Catalog#11074-H18H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7F11F5D2
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PRMT5 / SKB1 (rh PRMT5 / SKB1; Catalog#11074-H18H; NP_006128.1; Ala 2-Leu 637). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PRMT5 / SKB1 Antibody Usage Guide

• Specificity: Human PRMT5 / SKB1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human PRMT5 in WB. Using a DAB detection system, the detection limit for Human PRMT5 is approximately 8 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PRMT5. The detection limit for Human PRMT5 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PRMT5 / SKB1 Antibody Background

Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation, signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family, and exists as at least homodimers and homotetramers, or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development, as well as histone H3 'Arg-8', which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionall.

References

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4. Pal, S., et al., 2004, Mol. Cell. Biol. 24: 9630-9645.
5. Herrmann, FJ. et al., 2009, Cell Sci. 122 (Pt 5): 667-77.