Human PRL Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10275-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000948.3

cDNA Size:    684

Synonym:   PRL

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens prolactin DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 46 C/G resulting in the amino acid Leu substitution by Val.

Shipping

10 μg Plasmid DNA on Whatman FTA elute card Whatman Cat. No.: WB120410.
The product will be shipped at room temperature and will use 3-5 days on road typically.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGAACATCA AAGGATCGCC ATGGAAAGGG TCCCTCCTGC TGCTGGTGGT GTCAAACCTG
61       CTCCTGTGCC AGAGCGTGGC CCCCTTGCCC ATCTGTCCCG GCGGGGCTGC CCGATGCCAG
121      GTGACCCTTC GAGACCTGTT TGACCGCGCC GTCGTCCTGT CCCACTACAT CCATAACCTC
181      TCCTCAGAAA TGTTCAGCGA ATTCGATAAA CGGTATACCC ATGGCCGGGG GTTCATTACC
241      AAGGCCATCA ACAGCTGCCA CACTTCTTCC CTTGCCACCC CCGAAGACAA GGAGCAAGCC
301      CAACAGATGA ATCAAAAAGA CTTTCTGAGC CTGATAGTCA GCATATTGCG ATCCTGGAAT
361      GAGCCTCTGT ATCATCTGGT CACGGAAGTA CGTGGTATGC AAGAAGCCCC GGAGGCTATC
421      CTATCCAAAG CTGTAGAGAT TGAGGAGCAA ACCAAACGGC TTCTAGAGGG CATGGAGCTG
481      ATAGTCAGCC AGGTTCATCC TGAAACCAAA GAAAATGAGA TCTACCCTGT CTGGTCGGGA
541      CTTCCATCCC TGCAGATGGC TGATGAAGAG TCTCGCCTTT CTGCTTATTA TAACCTGCTC
601      CACTGCCTAC GCAGGGATTC ACATAAAATC GACAATTATC TCAAGCTCCT GAAGTGCCGA
661      ATCATCCACA ACAACAACTG CTAA

Quality Control: cDNA sequencing results is available upon request.