Mouse PKC nu/PRKD3 Gene cDNA Clone / ORF Clone

PRKD3 cDNA Clone Datasheet

PRKD3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_029239.3
• cDNA Size: 2670
• Gene Synonym: PKD3; Pkcnu; Prkcn; MGC47171; 4930557O20Rik; 5730497N19Rik; Prkd3
• Source: Mouse

PRKD3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protein kinase D3 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 699 G/C，993 G/C，1056 T/C，2367 G/A and 2457 A/T not causing the amino acid variation.

PRKD3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRKD3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRKD3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTGCAA ATAATTCCCC TCCATCAGCC CAGAAATCTG TGTTCCCTGC AACTGTTTCT
61       GCTGTGCTTC CAGCTCCTTC TCCGTGTTCA AGTCCCAAAA CAGGACTCTC AGCTCGGCTC
121      TCTAATGGCA GCTTCAGTGC CCCGTCACTC ACAAACTCTA GAGGCTCAGT GCACACAGTT
181      TCATTTCTAC TTCAGATTGG CCTCACACGA GAGAGTGTTA CCATCGAGGC CCAGGAACTG
241      TCTCTCTCTG CTGTCAAAGA CCTCGTGTGC TCCATTGTTT ATCAGAAGTT TCCAGAGTGT
301      GGATTCTTTG GCATGTATGA TAAAATTCTT CTCTTTCGCC ACGACATGAA CTCAGAAAAC
361      ATTTTGCAGC TGATTACCTC AGCAGATGAA ATACATGAAG GGGACCTGGT AGAAGTTGTC
421      CTGTCAGCTT TGGCCACAGT GGAAGACTTC CAGATCCGCC CTCACGCTCT CTATGTACAT
481      TCTTACAAAG CACCTACTTT TTGTGATTAC TGTGGTGAAA TGCTCTGGGG ACTGGTACGT
541      CAAGGATTAA AATGTGAAGG CTGTGGATTA AATTATCATA AACGATGTGC CTTCAAGATT
601      CCAAATAACT GCAGTGGAGT AAGAAAGAGA CGTCTGTCAA ATGTATCTCT GCCAGGACCT
661      GGCCTCTCAG TTCCAAGACC CCTGCAGCCT GAGTGTGTCC CCCTTCTCAG TGAAGAGTCA
721      CATACTCACC AAGAGCCCAG TAAGAGAATC CCGTCGTGGA GTGGTCGTCC GATCTGGATG
781      GAAAAGATGG TAATGTGCAG GGTCAAAGTC CCTCACACAT TTGCTGTCCA CTCTTATGGC
841      CGCCCGACCA TATGCCAGTA CTGCAAGCGC CTGCTGAAAG GCCTCTTCCG CCAAGGGATG
901      CAGTGTAAAG ATTGCAAATT CAACTGCCAT AAACGCTGTG CATCCAAAGT ACCAAGAGAC
961      TGCCTTGGCG AAGTTACTTT CAATGGAGAG CCCTGCAGCG TGGGGACTGA TGCAGACATG
1021     CCCATGGATA TTGACAGTAG TGATGTGAAC AGTGACGGCA GTAGGGGCTT GGATGACTCA
1081     GAGGAGCCGT CCCCGCCCGA AGACAAGATG TTCTTCCTCG ACCCGACTGA TCTCGACGTG
1141     GAGCGAGATG AGGAGACTGT TAAGACAATC AGTCCATCCA CCAGCAATAA TATCCCGCTG
1201     ATGAGGGTGG TGCAGTCCAT CAAGCACACA AAGAGGAGGA GCAGCACAGT TGTGAAGGAA
1261     GGGTGGATGG TCCACTACAC CAGCAGGGAC AACCTGAGAA AGAGACATTA TTGGAGACTT
1321     GACAGTAAAT GTCTAACATT GTTTCAAAAT GAATCTGGAT CAAAGTATTA TAAGGAAATT
1381     CCACTTTCAG AAATTCTCCG TGTATCTTCA CCGCAAGATT TCACCAGCAT TTCACAAGGC
1441     AGTAACCCAC ACTGTTTTGA AATCATCACC GATACCGTGG TATACTTTGT TGGTGAGAAC
1501     AATGGGAGCA GCTCTCATAA TCCTGTTCTC GCTGCCACTG GAGTGGGACT AGATGTGGCG
1561     CAGAGCTGGG AAAAAGCAAT TCGCCAGGCC CTCATGCCTG TCACTCCTCA AGCAAGTGTT
1621     TGCACTTCTC CGGGGCAAGG GAAGGACCAC AATCTGGCTA CCAGTATCTC CGTGTCTAAC
1681     TGTCAGGTTC AGGAGAATGT CGACATCAGC AGCGTTTACC AGATCTTTGC GGACGAGGTG
1741     CTTGGTTCTG GCCAGTTTGG CATTGTTTAT GGAGGAAAAC ACAGAAAGAC GGGAAGGGAT
1801     GTGGCTATTA AAGTGATTGA TAAAATGAGG TTCCCCACAA AGCAAGAGAG TCAGCTCCGG
1861     AACGAAGTGG CCATTTTACA GAATCTGCAC CATCCTGGGA TTGTAAACCT GGAGTGTATG
1921     TTTGAAACCC CAGAACGAGT CTTCGTGGTG ATGGAAAAGC TCCATGGAGA TATGTTGGAA
1981     ATGATTCTGT CCAGTGAAAA GAGTCGACTT CCAGAACGGA TTACCAAATT CATGGTCACA
2041     CAGATACTTG TTGCCTTGAG GAATTTACAT TTCAAGAATA TTGTGCACTG TGATTTAAAG
2101     CCCGAAAATG TGCTGCTTGC ATCCGCAGAG CCATTTCCTC AGGTGAAGCT GTGTGACTTT
2161     GGGTTTGCCC GCATCATTGG TGAGAAGTCG TTCCGGAGGT CAGTGGTAGG AACTCCAGCC
2221     TACTTAGCCC CTGAGGTTCT CAGGAGCAAA GGCTACAACC GTTCTCTAGA CATGTGGTCG
2281     GTGGGCGTCA TCGTTTATGT GAGCCTCAGT GGCACATTCC CATTCAATGA AGATGAAGAT
2341     ATAAATGACC AAATCCAAAA CGCTGCATTT ATGTACCCAC CAAATCCATG GCGAGAAATT
2401     TCCAGTGAAG CAATTGACTT GATAAACAAC TTGCTTCAAG TGAAGATGAG AAAACGTTAC
2461     AGTGTTGACA AATCTCTCAG TCATCCTTGG CTACAGGATT ATCAGACTTG GCTTGACCTC
2521     AGAGAATTTG AAACTCGCAT TGGAGAACGT TACATTACAC ACGAAAGTGA TGATGCTCGC
2581     TGGGAAATAC ACGCATACAC GCACAATCTG GAATACCCAA AGCATTTCAT TATGGCCCCC
2641     AACCCAGACG ACATGGAGGA GGATCCTTAG

 

Quality Control:cDNA sequencing results is available upon request.