Mouse PRKCB Gene cDNA Clone / ORF Clone

PRKCB cDNA Clone Datasheet

PRKCB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008855.2
• cDNA Size: 2022
• Gene Synonym: Pkcb; Prkcb1; Prkcb2; PKC-Beta; A130082F03Rik; Prkcb
• Source: Mouse

PRKCB cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protein kinase C, beta DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

PRKCB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRKCB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRKCB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGACC CGGCTGCGGG GCCGCCGCCG AGCGAGGGCG AGGAGAGCAC AGTGCGCTTC
61       GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC
121      GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC
181      GGGAAGCAGG GATTCCAGTG TCAAGTCTGC TGCTTTGTTG TACACAAGCG CTGCCATGAG
241      TTCGTCACGT TCTCCTGCCC CGGTGCGGAC AAGGGCCCGG CCTCTGATGA CCCCCGGAGC
301      AAACACAAGT TTAAGATCCA CACGTACTCC AGTCCTACCT TCTGTGACCA CTGTGGATCG
361      CTGCTGTATG GACTTATTCA CCAGGGGATG AAATGCGACA CCTGTATGAT GAACGTGCAC
421      AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACTG ACCACACGGA ACGCCGCGGC
481      CGCATCTATA TCCAGGCCCA CATCGACAGA GAGGTTCTCA TTGTTGTTGT AAGAGATGCT
541      AAAAATCTGG TACCTATGGA CCCCAATGGC TTGTCAGATC CCTACGTTAA ACTGAAACTG
601      ATCCCTGATC CCAAAAGTGA GAGCAAACAG AAGACCAAGA CCATCAAGTG TTCCCTCAAT
661      CCAGAGTGGA ATGAAACCTT CAGATTTCAG CTGAAGGAAT CAGACAAAGA CAGAAGACTG
721      TCTGTAGAGA TCTGGGATTG GGACCTGACC AGCAGGAATG ACTTCATGGG ATCTCTGTCA
781      TTTGGGATTT CAGAACTACA GAAAGCCGGT GTGGATGGCT GGTTCAAGTT ACTAAGCCAG
841      GAAGAGGGTG AATACTTCAA TGTGCCCGTG CCGCCCGAAG GAAGCGAGGG CAATGAAGAG
901      CTGCGGCAGA AGTTTGAGAG AGCCAAGATT GGCCAAGGTA CCAAGGCTCC AGAAGAAAAG
961      ACAGCTAACA CTATCTCCAA ATTTGACAAC AATGGCAACA GGGACCGGAT GAAACTGACC
1021     GATTTTAACT TCCTGATGGT GCTGGGGAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG
1081     CGGAAGGGTA CAGATGAACT CTATGCCGTG AAGATCCTGA AGAAGGACGT GGTGATCCAA
1141     GATGACGATG TGGAGTGCAC CATGGTGGAG AAGCGGGTGC TGGCCCTGCC TGGGAAGCCC
1201     CCGTTCCTGA CTCAGCTCCA TTCCTGCTTC CAGACCATGG ACCGCCTGTA CTTTGTGATG
1261     GAGTATGTGA ACGGGGGTGA CCTCATGTAC CACATCCAAC AAGTTGGCCG TTTCAAGGAG
1321     CCCCATGCTG TATTTTACGC CGCAGAGATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG
1381     GGCATTATTT ACCGCGACCT GAAACTTGAC AACGTGATGC TGGATTCTGA GGGGCACATC
1441     AAAATCGCTG ACTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC AACCAAGACA
1501     TTCTGTGGCA CTCCGGACTA CATTGCTCCA GAGATCATTG CTTATCAGCC CTACGGGAAG
1561     TCTGTGGACT GGTGGGCATT TGGAGTCCTG CTGTATGAAA TGTTGGCTGG CCAGGCACCG
1621     TTTGAAGGGG AGGATGAGGA TGAACTCTTC CAGTCAATCA TGGAACACAA TGTGGCGTAT
1681     CCCAAGTCCA TGTCCAAGGA AGCCGTGGCA ATCTGCAAAG GGCTAATGAC CAAACACCCA
1741     GGCAAGCGCC TGGGTTGTGG GCCTGAAGGG GAACGAGACA TTAAGGAGCA TGCGTTTTTC
1801     CGGTATATTG ACTGGGAGAA ACTCGAACGC AAGGAGATTC AGCCACCTTA TAAACCAAAA
1861     GCTTGTGGGC GAAACGCTGA AAACTTCGAC CGGTTTTTCA CCCGCCATCC ACCAGTCCTA
1921     ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC
1981     TTTGTTAACT CTGAATTTTT AAAACCTGAA GTCAAGAGCT AA

 

Quality Control:cDNA sequencing results is available upon request.