AMPK (G1/B2/A1) Heterotrimer Protein

AMPK (G1/B2/A1) Heterotrimer Protein Datasheet

AMPK (G1/B2/A1) Heterotrimer Protein Product Information

• Protein name: AMPK, 5' AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 1 catalytic subunit, beta 2 non-catalytic subunit and gamma 1 non-catalytic subunit.
• Protein Construction: A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2.; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
• Source: Human
• Expression Host: Baculovirus-Insect cells

AMPK (G1/B2/A1) Heterotrimer Protein QC Testing

• Purity: > 94 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met & Met & His
• Molecular Mass: The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris ,200mM NaCl,2mM GSH,1mM EDTA ,1mM DTT，10%gly, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: AMPK (G1/B2/A1) Heterotrimer protein

AMPK (G1/B2/A1) Heterotrimer Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

AMPK (G1/B2/A1) Heterotrimer Protein Description

AMP-activated protein kinase (AMPK) is an important metabolic stress-sensing protein kinase induced by AMP. It can be detected in a number of tissues, including the liver, brain, and skeletal muscle. AMPK is a heterotrimer comprised by three proteins (α, β, and γ subunits) that together make a functional enzyme, conserved from yeast to humans. Seven known genes encode the subunit isoforms (α1, α2, β1, β2, γ1, γ2, γ3) and all are expressed in the heart. The most common isoforms expressed in most cells are the α1(PRKAA1), β1(PRKAB1), and γ1(PRKAG1) isoforms. AMPK induces a cascade of events within cells in response to the ever changing energy charge of the cell. Mammalian AMPK is a stable alphabetagamma heterotrimer comprising a catalytic alpha (PRKAA) and two non-catalytic subunits, beta (PRKAB) and gamma (PRKAG). AMPK activation results in the stimulation of hepatic fatty acid oxidation and ketogenesis, inhibition of cholesterol synthesis, lipogenesis, and triglyceride synthesis, inhibition of adipocyte lipolysis and lipogenesis, stimulation of skeletal muscle fatty acid oxidation and muscle glucose uptake, and modulation of insulin secretion by pancreatic beta-cells.

References

1. Adams J. et al., 2004, Protein Sci. 13 (1): 155-65.
2. Stapleton D. et al., 1996, J Biol Chem. 271 (2): 611-4.
3. Hallows KR. et al., 2009, Am J Physiol. 296 (4): C672-81.
4. Winder WW. et al., 1999, AmJPhysiol. 277 (1Pt1): E1-10.