Human PRKACB Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12272-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PRKACB cDNA Clone Datasheet

PRKACB cDNA Clone Product Information

Gene Bank Ref.ID:	BC016285
cDNA Size:	774
Gene Synonym:	PKACB; PRKACB
Source:	Human

PRKACB cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protein kinase, cAMP-dependent, catalytic, beta DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PRKACB cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRKACB cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRKACB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGAACG CGGCGACCGC CAAGAAAGGC AGCGAGGTGG AGAGCGTGAA AGAGTTTCTA
61       GCCAAAGCCA AAGAAGACTT TTTGAAAAAA TGGGAGAATC CAACTCAGAA TAATGCCGGA
121      CTTGAAGATT TTGAAAGGAA AAAAACCCTT GGAACAGGTT CATTTGGAAG AGTCATGTTG
181      GTAAAACACA AAGCCACTGA ACAGTATTAT GCCATGAAGA TCTTAGATAA GCAGAAGGTT
241      GTTAAACTGA AGCAAATAGA GCATACTTTG AATGAGAAAA GAATATTACA GGCAGTGAAT
301      TTTCCTTTCC TTGTTCGACT GGAGTATGCT TTTAAGGATA ATTCTAATTT ATACATGGTT
361      ATGGAATATG TCCCTGGGGG TGAAATGTTT TCACATCTAA GAAGAATTGG AAGGTTCAGT
421      GAGCCCCATG CACGGTTCTA TGCAGCTCAG ATAGTGCTAA CATTCGAGTA CCTCCATTCA
481      CTAGACCTCA TCTACAGAGA TCTAAAACCT GAAAATCTCT TAATTGACCA TCAAGGCTAT
541      ATCCAGGTCA CAGACTTTGG GTTTGCCAAA AGAGTTAAAG GCAGAACTTG GACATTATGT
601      GGAACTCCAG AGTATTTGGC TCCAGAAATA ATTCTCAGCA AGGGCTACAA TAAGGCAGTG
661      GATTGGTGGG CATTAGGAGT GCTAATCTAT GAAATGGCAG CTGGCTATCC CCCATTCTTT
721      GCAGACCAAC CAATTCAGAT TTATGAAAAG ATTGTTTCTG GAAAGAACTT TTGA

Quality Control:cDNA sequencing results is available upon request.