Human PRKACB Gene cDNA Clone / ORF Clone
|Catalog||Vector (Price)||Quantity||In Stock||Operation|
PRKACB cDNA Clone Datasheet
PRKACB cDNA Clone Product Information
|Gene Bank Ref.ID:||BC016285|
PRKACB cDNA Clone Description
ORF Clone of Homo sapiens protein kinase, cAMP-dependent, catalytic, beta DNA.
|Vector:||Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
|Sequence Description:||Identical with the Gene Bank Ref.ID sequence.|
PRKACB cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
PRKACB cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
PRKACB cDNA Clone Sequence and Quality Control
1 ATGGGGAACG CGGCGACCGC CAAGAAAGGC AGCGAGGTGG AGAGCGTGAA AGAGTTTCTA 61 GCCAAAGCCA AAGAAGACTT TTTGAAAAAA TGGGAGAATC CAACTCAGAA TAATGCCGGA 121 CTTGAAGATT TTGAAAGGAA AAAAACCCTT GGAACAGGTT CATTTGGAAG AGTCATGTTG 181 GTAAAACACA AAGCCACTGA ACAGTATTAT GCCATGAAGA TCTTAGATAA GCAGAAGGTT 241 GTTAAACTGA AGCAAATAGA GCATACTTTG AATGAGAAAA GAATATTACA GGCAGTGAAT 301 TTTCCTTTCC TTGTTCGACT GGAGTATGCT TTTAAGGATA ATTCTAATTT ATACATGGTT 361 ATGGAATATG TCCCTGGGGG TGAAATGTTT TCACATCTAA GAAGAATTGG AAGGTTCAGT 421 GAGCCCCATG CACGGTTCTA TGCAGCTCAG ATAGTGCTAA CATTCGAGTA CCTCCATTCA 481 CTAGACCTCA TCTACAGAGA TCTAAAACCT GAAAATCTCT TAATTGACCA TCAAGGCTAT 541 ATCCAGGTCA CAGACTTTGG GTTTGCCAAA AGAGTTAAAG GCAGAACTTG GACATTATGT 601 GGAACTCCAG AGTATTTGGC TCCAGAAATA ATTCTCAGCA AGGGCTACAA TAAGGCAGTG 661 GATTGGTGGG CATTAGGAGT GCTAATCTAT GAAATGGCAG CTGGCTATCC CCCATTCTTT 721 GCAGACCAAC CAATTCAGAT TTATGAAAAG ATTGTTTCTG GAAAGAACTT TTGA
Quality Control:cDNA sequencing results is available upon request.