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人 PRKACA 基因全长ORF克隆

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Human PRKACA cDNA Clone Product Information
NCBI RefSeq:NM_002730.3
RefSeq ORF Size:1056bp
cDNA Description:Full length Clone DNA of Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha.
Gene Synonym:PKACA, MGC48865, MGC102831, PRKACA
Species:Human
Vector:pMD18-T Vector
Plasmid:pMD-PRKACA
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 618T/C resulting in the amino acid Leu substitution by Pro.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Vector Information

pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which multiple cloning sites as shown below. The pMD18-T Vector is 2.6kb in size and contains the amplicin resistance gene for selection. The coding sequence was inserted by TA cloning at site 425.

pMD18-T vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

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Catalog: HG11544-M
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