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Human PRKACA Gene cDNA Clone (full-length ORF Clone)
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Human PRKACA Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG11544-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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PRKACA cDNA Clone Datasheet

 

PRKACA cDNA Clone Product Information

Gene Bank Ref.ID: NM_002730.3

cDNA Size:

1056

Gene Synonym:

PKACA; MGC48865; MGC102831; PRKACA

Source: Human

PRKACA cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha DNA.

Vector: Cloning Vector: pMD18-T Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 618T/C resulting in the amino acid Leu substitution by Pro.

PRKACA cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRKACA cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PRKACA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCAACG CCGCCGCCGC CAAGAAGGGC AGCGAGCAGG AGAGCGTGAA AGAATTCTTA
61       GCCAAAGCCA AAGAAGATTT TCTTAAAAAA TGGGAAAGTC CCGCTCAGAA CACAGCCCAC
121      TTGGATCAGT TTGAACGAAT CAAGACCCTC GGCACGGGCT CCTTCGGGCG GGTGATGCTG
181      GTGAAACACA AGGAGACCGG GAACCACTAT GCCATGAAGA TCCTCGACAA ACAGAAGGTG
241      GTGAAACTGA AACAGATCGA ACACACCCTG AATGAAAAGC GCATCCTGCA AGCTGTCAAC
301      TTTCCGTTCC TCGTCAAACT CGAGTTCTCC TTCAAGGACA ACTCAAACTT ATACATGGTC
361      ATGGAGTACG TGCCCGGCGG GGAGATGTTC TCACACCTAC GGCGGATCGG AAGGTTCAGT
421      GAGCCCCATG CCCGTTTCTA CGCGGCCCAG ATCGTCCTGA CCTTTGAGTA TCTGCACTCG
481      CTGGATCTCA TCTACAGGGA CCTGAAGCCG GAGAATCTGC TCATTGACCA GCAGGGCTAC
541      ATTCAGGTGA CAGACTTCGG TTTCGCCAAG CGCGTGAAGG GCCGCACTTG GACCTTGTGC
601      GGCACCCCTG AGTACCCGGC CCCTGAGATT ATCCTGAGCA AAGGCTACAA CAAGGCCGTG
661      GACTGGTGGG CCCTGGGGGT TCTTATCTAT GAAATGGCCG CTGGCTACCC GCCCTTCTTC
721      GCAGACCAGC CCATCCAGAT CTATGAGAAG ATCGTCTCTG GGAAGGTGCG CTTCCCTTCC
781      CACTTCAGCT CTGACTTGAA GGACCTGCTG CGGAACCTCC TGCAGGTAGA TCTCACCAAG
841      CGCTTTGGGA ACCTCAAGAA TGGGGTCAAC GATATCAAGA ACCACAAGTG GTTTGCCACA
901      ACTGACTGGA TTGCCATCTA CCAGAGGAAG GTGGAAGCTC CCTTCATACC AAAGTTTAAA
961      GGCCCTGGGG ATACGAGTAA CTTTGACGAC TATGAGGAAG AAGAAATCCG GGTCTCCATC
1021     AATGAGAAGT GTGGCAAGGA GTTTTCTGAG TTTTAG

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"