Human PRKAB2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12032-G	Cloning vector ($125) pCMV / hygro ($325) pCMV / hygro - HA ($325) pCMV / hygro - His ($325) pCMV / hygro - Myc ($325) pCMV / hygro - FLAG ($325)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PRKAB2 cDNA Clone Datasheet

PRKAB2 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_005399
cDNA Size:	819
Gene Synonym:	MGC61468; PRKAB2
Source:	Human

PRKAB2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protein kinase, AMP-activated, beta 2 non-catalytic subunit DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PRKAB2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRKAB2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

PRKAB2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAAACA CCACCAGCGA CCGGGTGTCC GGGGAGCGCC ACGGCGCCAA GGCTGCACGC
61       TCCGAGGGCG CAGGCGGCCA TGCCCCGGGG AAGGAGCACA AGATCATGGT GGGGAGTACG
121      GACGACCCCA GCGTGTTCAG CCTCCCTGAC TCCAAGCTCC CTGGGGACAA AGAGTTTGTA
181      TCATGGCAGC AGGATTTGGA GGACTCCGTA AAGCCCACAC AGCAGGCCCG GCCCACTGTT
241      ATCCGCTGGT CTGAAGGAGG CAAGGAGGTC TTCATCTCTG GGTCCTTCAA CAATTGGAGC
301      ACCAAGATTC CACTGATTAA GAGCCATAAT GACTTTGTTG CCATCCTGGA CCTCCCTGAG
361      GGAGAGCACC AATACAAGTT CTTTGTGGAT GGACAGTGGG TTCATGATCC ATCAGAGCCT
421      GTGGTTACCA GTCAGCTTGG CACAATTAAC AATTTGATCC ATGTCAAGAA ATCTGATTTT
481      GAGGTGTTCG ATGCTTTAAA GTTAGATTCT ATGGAAAGTT CTGAGACATC TTGTAGAGAC
541      CTTTCCAGCT CACCCCCAGG GCCTTATGGT CAAGAAATGT ATGCGTTTCG ATCTGAGGAA
601      AGATTCAAAT CCCCACCCAT CCTTCCTCCT CATCTACTTC AAGTTATTCT TAACAAAGAC
661      ACTAATATTT CTTGTGACCC AGCCTTACTC CCTGAGCCCA ACCATGTTAT GCTGAACCAT
721      CTCTATGCAT TGTCCATTAA GGACAGTGTG ATGGTCCTTA GCGCAACCCA TCGCTACAAG
781      AAGAAGTATG TTACTACTCT GCTATACAAG CCCATTTGA

Quality Control:cDNA sequencing results is available upon request.