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Human PRK2/Prkcl2 Gene ORF cDNA clone in cloning vector

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Human PRK2/Prkcl2 cDNA Clone Product Information
NCBI RefSeq:NM_006256.2
RefSeq ORF Size:2955bp
cDNA Description:Full length Clone DNA of Homo sapiens protein kinase N2.
Gene Synonym:PAK2, PRK2, Pak-2, PRKCL2, PRO2042, MGC71074, MGC150606, PKN2
Species:Human
Vector:pMD18-T Simple Vector
Plasmid:pMD-PRK2
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for two point mutations: 421 T/C and 1677 T/C not causing the amino acid variation.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
PRK2/Prkcl2neucleotide sequence and amino acid sequence
 
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human PRK2/Prkcl2 Gene ORF cDNA clone in cloning vector on other vectors
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10536-ACG$325
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG10536-ACR$325
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG10536-ANG$325
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG10536-ANR$325
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10536-CF$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-His tagHG10536-CH$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-Myc tagHG10536-CM$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-HA tagHG10536-CY$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10536-M-F$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmidHG10536-M-N$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, N-Flag tagHG10536-NF$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, N-His tagHG10536-NH$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, N-Myc tagHG10536-NM$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmid, N-HA tagHG10536-NY$295
Human PRK2/Prkcl2 Gene ORF cDNA clone expression plasmidHG10536-UT$295
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