Human PRH1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13709-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PRH1 cDNA Clone Datasheet

PRH1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC133676
cDNA Size:	501
Gene Synonym:	MGC74956; PA; PRH1
Source:	Human

PRH1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens proline-rich protein HaeIII subfamily 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 124 C/A resulting in the amino acid Leu substitution by Ile.

PRH1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRH1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRH1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTCTGA TTCTGCTGTC AGTGGCCCTG CTGGCCTTCA GCTCAGCTCA GGATTTAAAT
61       GAAGATGTCA GCCAGGAAGA TGTTCCCCTC GTAATATCAG ATGGAGGAGA CTCTGAGCAG
121      TTCATAGATG AGGAGCGTCA GGGACCACCT TTGGGAGGAC AGCAATCTCA ACCCTCTGCT
181      GGTGATGGGA ACCAGGATGA TGGCCCTCAG CAGGGACCAC CCCAACAAGG AGGCCAGCAG
241      CAACAAGGTC CACCACCTCC TCAGGGAAAG CCACAAGGAC CACCCCAACA GGGAGGCCAT
301      CCCCCTCCTC CTCAAGGAAG GCCACAAGGA CCACCCCAAC AGGGAGGCCA TCCCCGTCCT
361      CCTCGAGGAA GGCCACAAGG ACCACCCCAA CAGGGAGGCC ATCAGCAAGG TCCTCCCCCA
421      CCTCCTCCTG GAAAGCCCCA GGGACCACCT CCCCAAGGGG GCCGCCCACA AGGACCTCCA
481      CAGGGGCAGT CTCCTCAGTA A

Quality Control:cDNA sequencing results is available upon request.