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Human PRF1 Gene cDNA Clone (full-length ORF Clone)
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Human PRF1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG15267-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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PRF1 cDNA Clone Datasheet

 

PRF1 cDNA Clone Product Information

Gene Bank Ref.ID: BC063043

cDNA Size:

1668

Gene Synonym:

P1, PFP, FLH2, PFN1, HPLH2

Source: Human

PRF1 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens perforin 1 (pore forming protein) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

PRF1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRF1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGCCC GTCTGCTCCT CCTGGGCATC CTTCTCCTGC TGCTGCCCCT GCCCGTCCCT
61       GCCCCGTGCC ACACAGCCGC ACGCTCAGAG TGCAAGCGCA GCCACAAGTT CGTGCCTGGT
121      GCATGGCTGG CCGGGGAGGG TGTGGACGTG ACCAGCCTCC GCCGCTCGGG CTCCTTCCCA
181      GTGGACACAC AAAGGTTCCT GCGGCCCGAC GGCACCTGCA CCCTCTGTGA AAATGCCCTA
241      CAGGAGGGCA CCCTCCAGCG CCTGCCTCTG GCGCTCACCA ACTGGCGGGC CCAGGGCTCT
301      GGCTGCCAGC GCCATGTAAC CAGGGCCAAA GTCAGCTCCA CTGAAGCTGT GGCCCGGGAT
361      GCGGCTCGTA GCATCCGCAA CGACTGGAAG GTCGGGCTGG ACGTGACTCC TAAGCCCACC
421      AGCAATGTGC ATGTGTCTGT GGCCGGCTCA CACTCACAGG CAGCCAACTT TGCAGCCCAG
481      AAGACCCACC AGGACCAGTA CAGCTTCAGC ACTGACACGG TGGAGTGCCG CTTCTACAGT
541      TTCCATGTGG TACACACTCC CCCGCTGCAC CCTGACTTCA AGAGGGCCCT CGGGGACCTG
601      CCCCACCACT TCAACGCCTC CACCCAGCCC GCCTACCTCA GGCTTATCTC CAACTACGGC
661      ACCCACTTCA TCCGGGCTGT GGAGCTGGGT GGCCGCATAT CGGCCCTCAC TGCCCTGCGC
721      ACCTGCGAGC TGGCCCTGGA AGGGCTCACG GACAACGAGG TGGAGGACTG CCTGACTGTC
781      GAGGCCCAGG TCAACATAGG CATCCACGGC AGCATCTCTG CCGAAGCCAA GGCCTGTGAG
841      GAGAAGAAGA AGAAGCACAA GATGACGGCC TCCTTCCACC AAACCTACCG GGAGCGCCAT
901      TCGGAAGTGG TTGGCGGCCA TCACACCTCC ATTAACGACC TGCTGTTCGG GATCCAGGCC
961      GGGCCCGAGC AGTACTCAGC CTGGGTAAAC TCGCTGCCCG GCAGCCCTGG CCTGGTGGAC
1021     TACACCCTGG AACCCCTGCA CGTGCTGCTG GACAGCCAGG ACCCGCGGCG GGAGGCACTG
1081     AGGAGGGCCC TGAGTCAGTA CCTGACGGAC AGGGCTCGCT GGAGGGACTG CAGCCGGCCG
1141     TGCCCACCAG GGCGGCAGAA GAGCCCCCGA GACCCATGCC AGTGTGTGTG CCATGGCTCA
1201     GCGGTCACCA CCCAGGACTG CTGCCCTCGG CAGAGGGGCC TGGCCCAGCT GGAGGTGACC
1261     TTCATCCAAG CATGGGGCCT GTGGGGGGAC TGGTTCACTG CCACGGATGC CTATGTGAAG
1321     CTCTTCTTTG GTGGCCAGGA GCTGAGGACG AGCACCGTGT GGGACAATAA CAACCCCATC
1381     TGGTCAGTGC GGCTGGATTT TGGGGATGTG CTCCTGGCCA CAGGGGGGCC CCTGAGGTTG
1441     CAGGTCTGGG ATCAGGACTC TGGCAGGGAC GATGACCTCC TTGGCACCTG TGATCAGGCT
1501     CCCAAGTCTG GTTCCCATGA GGTGAGATGC AACCTGAATC ATGGCCACCT AAAATTCCGC
1561     TATCATGCCA GGTGCTTGCC CCACCTGGGA GGAGGCACCT GCCTGGACTA TGTCCCCCAA
1621     ATGCTTCTGG GGGAGCCTCC AGGAAACCGG AGTGGGGCCG TGTGGTGA

 

Quality Control:cDNA sequencing results is available upon request.


 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"