PRDM2 / KMT8 Protein (GST Tag)

PR Domain Zinc Finger Protein 2 ( PRDM2 / KMT8 ) Protein

PRDM2 / KMT8 Protein Product Information

• Synonym: RP5-1177E19.1 , HUMHOXY1, KMT8, MTB-ZF, RIZ, RIZ1, RIZ2, PRDM2
• Protein Construction: A DNA sequence encoding the N-terminal segment of human PRDM2 ( NP_036363.2 ) ( Met 1 - Ala 200 ), containing the SET domian, was fused with the GST tag at the N-terminus
• Source: Human
• Expression Host: E.coli

PRDM2 / KMT8 Protein QC Testing

• Purity: > 86 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PRDM2 / GST chimera consists of 432 amino acids and predicts a molecular mass of 49.6 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 20mM Tris , 150mM NaCl , 0.5mM DTT , 0.5mM GSH , pH 8.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PRDM2 protein

PRDM2 / KMT8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PRDM2 / KMT8 Protein Description

PR domain containing 2, with ZNF domain (PRDM2), also known as zinc finger protein RIZ, is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain, which shares sequence homology with an independently described sequence motif, the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain, and is highly expressed in retinoblastoma cell lines and in brain tumors, as well as in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3, and is identified as a tumor suppressor. It is reported that intact PR( SET) sequence is required for tumor suppression functions, mutations in the PR domain caused activity reduction in human cancers. Also, S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition, PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3.

References

1. Buyes, I.M. et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92: 4467-4471.
2. Muraosa, Y. et al., 1996, Eur. J. Biochem. 235: 471-479.
3. Kim, K. et al., 2003, Cancer. Res. 63: 7619-7623.
4. Shapiro, V.S. et al., 1995, Gene. 163: 329-330.
5. Briknarova, K.et al.,2008,Biochem.Biophys.Res.Commun.366:807-813.