|Catalog||Size (Price)||Quantity||In Stock||Operation||Other Information|
PR Domain Zinc Finger Protein 2 ( PRDM2 / KMT8 ) Protein
PRDM2 / KMT8 Protein Price Inquiry ( Available Sizes )
- ≥1mg Bulk: Inquiring Price
PRDM2 / KMT8 Protein Product Information
RP5-1177E19.1 , HUMHOXY1, KMT8, MTB-ZF, RIZ, RIZ1, RIZ2, PRDM2
A DNA sequence encoding the N-terminal segment of human PRDM2 ( NP_036363.2 ) ( Met 1 - Ala 200 ), containing the SET domian, was fused with the GST tag at the N-terminus
PRDM2 / KMT8 Protein QC Testing
|Purity:||> 86 % as determined by SDS-PAGE||SDS-PAGE:
|Endotoxin:||Please contact us for more information.|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Met|
The recombinant human PRDM2 / GST chimera consists of 432 amino acids and predicts a molecular mass of 49.6 kDa as estimated in SDS-PAGE under reducing conditions
|Formulation:||Lyophilized from sterile 20mM Tris , 150mM NaCl , 0.5mM DTT , 0.5mM GSH , pH 8.0
PRDM2 / KMT8 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
PRDM2 / KMT8 Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|PRDM2/RIZ1/KMT8||Human||PRDM2/RIZ1/KMT8 Protein, Recombinant, With GST Tag||11216-H09E|
|PRDM2/RIZ1/KMT8||Human||PRDM2/RIZ1/KMT8 Protein, Recombinant||11216-HNCE|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||PRDM2/RIZ1 Antibody, Rabbit PAb||11216-RP01|
|WB, ELISA||PRDM2/RIZ1 Antibody, Rabbit PAb (Antigen Affinity Purified)||11216-RP02|
PRDM2 / KMT8 Protein Description
PR domain containing 2, with ZNF domain (PRDM2), also known as zinc finger protein RIZ, is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain, which shares sequence homology with an independently described sequence motif, the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain, and is highly expressed in retinoblastoma cell lines and in brain tumors, as well as in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3, and is identified as a tumor suppressor. It is reported that intact PR( SET) sequence is required for tumor suppression functions, mutations in the PR domain caused activity reduction in human cancers. Also, S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition, PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3.
- Buyes, I.M. et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92: 4467-4471.
- Muraosa, Y. et al., 1996, Eur. J. Biochem. 235: 471-479.
- Kim, K. et al., 2003, Cancer. Res. 63: 7619-7623.
- Shapiro, V.S. et al., 1995, Gene. 163: 329-330.
- Briknarova, K.et al.,2008,Biochem.Biophys.Res.Commun.366:807-813.