Anti-RIZ1 Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human PRDM2 protein (Catalog#11216-H09E)
Produced in rabbits immunized with purified, recombinant Human PRDM2 (rh PRDM2; Catalog#11216-H09E; NP_036363.2; Met 1-Ala 200). PRDM2 specific IgG was purified by human PRDM2 affinity chromatography
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-RIZ1 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-RIZ1 Antibody (Rabbit Polyclonal antibody) Images
Immunochemical staining of human PRDM2 in human glioblastoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Immunochemical staining of human PRDM2 in human astrocytoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-RIZ1 Antibody Alternative Names
Anti-HUMHOXY1 Antibody;Anti-KMT8 Antibody;Anti-MTB-ZF Antibody;Anti-RIZ Antibody;Anti-RIZ1 Antibody;Anti-RIZ2 Antibody
RIZ1 Background Information
PR domain containing 2, with ZNF domain (PRDM2), also known as zinc finger protein RIZ, is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain, which shares sequence homology with an independently described sequence motif, the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain, and is highly expressed in retinoblastoma cell lines and in brain tumors, as well as in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3, and is identified as a tumor suppressor. It is reported that intact PR( SET) sequence is required for tumor suppression functions, mutations in the PR domain caused activity reduction in human cancers. Also, S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition, PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3.
PR domain containing 2, with ZNF domain
Buyes, I.M. et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92: 4467-4471. Muraosa, Y. et al., 1996, Eur. J. Biochem. 235: 471-479. Kim, K. et al., 2003, Cancer. Res. 63: 7619-7623. Shapiro, V.S. et al., 1995, Gene. 163: 329-330. Briknarova, K.et al.,2008,Biochem.Biophys.Res.Commun.366:807-813.