Human PPY Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13358-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PPY cDNA Clone Datasheet

PPY cDNA Clone Product Information

Gene Bank Ref.ID:	BC032225
cDNA Size:	288
Gene Synonym:	PNP; PPY
Source:	Human

PPY cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens pancreatic polypeptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PPY cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PPY cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PPY cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCCG CACGCCTCTG CCTCTCCCTG CTGCTCCTGT CCACCTGCGT GGCTCTGTTA
61       CTACAGCCAC TGCTGGGTGC CCAGGGAGCC CCACTGGAGC CAGTGTACCC AGGGGACAAT
121      GCCACACCAG AGCAGATGGC CCAGTATGCA GCTGATCTCC GTAGATACAT CAACATGCTG
181      ACCAGGCCTA GGTATGGGAA AAGACACAAA GAGGACACGC TGGCCTTCTC GGAGTGGGGG
241      TCCCCGCATG CTGCTGTCCC CAGGGAGCTC AGCCCGCTGG ACTTATAA

Quality Control:cDNA sequencing results is available upon request.