Human PPTC7 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13800-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PPTC7 cDNA Clone Datasheet

PPTC7 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_139283.1
cDNA Size:	915
Gene Synonym:	TAPP2C; TA-PP2C; PPTC7
Source:	Human

PPTC7 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens PTC7 protein phosphatase homolog (S. cerevisiae)DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 867 A/G not causing the amino acid variation.

PPTC7 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PPTC7 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PPTC7 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCTCGG TCCTCTCGTA CGGGCGGCTG GTGGCCCGCG CCGTGCTCGG CGGCCTCTCG
61       CAGACCGACC CCAGGGCCGG CGGCGGCGGC GGCGGCGACT ACGGACTGGT GACGGCCGGC
121      TGCGGCTTCG GGAAGGACTT CCGTAAGGGC CTCCTCAAGA AGGGCGCGTG CTACGGGGAC
181      GACGCGTGCT TCGTGGCCCG GCACCGTTCC GCGGACGTGC TCGGGGTTGC AGATGGTGTA
241      GGAGGCTGGA GAGACTATGG AGTTGATCCA TCTCAATTCT CAGGGACTTT AATGCGGACG
301      TGTGAACGTT TAGTAAAAGA AGGACGGTTC GTACCTAGTA ATCCCATTGG AATTCTCACC
361      ACAAGCTACT GTGAGTTGCT GCAAAATAAA GTCCCTTTGC TCGGTAGCAG CACCGCCTGC
421      ATTGTGGTGC TGGACAGAAC CAGCCACCGC TTACACACAG CAAACCTGGG CGATTCAGGC
481      TTCCTGGTTG TCAGGGGTGG TGAAGTCGTG CACCGATCAG ATGAGCAGCA GCATTACTTC
541      AACACTCCAT TCCAGCTCTC AATCGCTCCC CCTGAAGCCG AGGGAGTCGT CTTGAGCGAC
601      AGTCCGGATG CTGCTGATAG CACGTCTTTC GATGTCCAGC TAGGAGACAT TATCCTGACG
661      GCAACAGATG GACTCTTTGA CAACATGCCT GATTATATGA TTCTTCAGGA GCTAAAAAAG
721      TTAAAGAATT CAAATTATGA GAGTATACAA CAGACTGCCA GAAGCATTGC TGAGCAAGCT
781      CATGAGCTGG CCTATGACCC AAATTATATG TCACCTTTTG CACAGTTTGC ATGTGACAAT
841      GGATTGAATG TGAGAGGTGG AAAGCCGGAT GACATCACCG TCCTTCTTTC AATAGTGGCT
901      GAGTATACAG ACTAG

Quality Control:cDNA sequencing results is available upon request.