Anti-PPM1G Antibody

Cat: 11245-RP02
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All PPM1G Reagents
CRO Services
Product recommended

All PPM1G Reagents
Anti-PPM1G Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PPM1G Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human PPM1G
Immunogen
Recombinant Human PPM1G protein (Catalog#11245-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human PPM1G (rh PPM1G; Catalog#11245-H07E; O15355; Met 317-Asp 546). PPM1G specific IgG was purified by Human PPM1G affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PPM1G Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PPM1G Antibody (Rabbit Polyclonal antibody) Images
Human PPM1G Western blot (WB) 13377

Anti-PPM1G rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: 292T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:59 kDa

Observed band size:80 kDa

(We are unsure as to the identity of these extra bands.)

Human PPM1G Immunoprecipitation(IP) 17688

PPM1G was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-PPM1G rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PPM1G rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 59 kDa

Observed band size: 72 kDa

Anti-PPM1G Antibody Alternative Names
Anti-PP2CG Antibody;Anti-PP2CGAMMA Antibody;Anti-PPP2CG Antibody
PPM1G Background Information

Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein which belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways.  PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.

Full Name
protein phosphatase, Mg2+/Mn2+ dependent, 1G
References
  • Travis S.M., et al., 1997, FEBS Lett. 412:415-9.
  • Molina H., et al., 2007, Proc. Natl. Acad. Sci. USA. 104: 2199-204.
  • Matsuoka S., et al., 2007, Science 316:1160-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PPM1G Antibody Rabbit Monoclonal WB,IHC-P,ICC/IF,IF 11245-R112
    Anti-PPM1G Antibody Rabbit Monoclonal WB,ELISA,IP 11245-R001
    Anti-PPM1G Antibody Rabbit Polyclonal ELISA 11245-RP01
    info info
    添加购物车成功! 添加购物车失败!