Human PPM1B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12224-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PPM1B cDNA Clone Datasheet

PPM1B cDNA Clone Product Information

Gene Bank Ref.ID:	BC012002
cDNA Size:	579
Gene Synonym:	PP2CB; PP2CBETA; PPC2BETAX; PP2C-beta-X; PPM1B
Source:	Human

PPM1B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protein phosphatase 1B (formerly 2C), magnesium-de DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PPM1B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PPM1B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PPM1B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTATTG TACTAGTTTG CTTTTCAAAT GCTCCCAAGG TCTCAGATGA AGCGGTGAAA
61       AAAGATTCAG AGTTGGATAA GCACTTGGAA TCACGGGTTG AAGAGATTAT GGAGAAGTCT
121      GGCGAGGAAG GAATGCCTGA TCTTGCCCAT GTCATGCGCA TCTTGTCTGC AGAAAATATC
181      CCAAATTTGC CTCCTGGGGG AGGTCTTGCT GGCAAGCGTA ATGTTATTGA AGCTGTTTAT
241      AGTAGACTGA ATCCACATAG AGAAAGTGAT GGGGCCTCCG ATGAAGCAGA GGAAAGTGGA
301      TCACAGGGAA AATTGGTGGA AGCTCTCAGG CAAATGAGAA TTAATCATAG GGGAAACTAC
361      CGACAACTTC TGGAGGAGAT GCTGACTAGT TACAGGCTAG CTAAAGTAGA GGGAGAAGAA
421      AGCCCTGCTG AACCAGCTGC CACAGCTACT TCTTCGAACA GTGATGCTGG AAACCCAGTG
481      ACAATGCAGG AAAGCCATAC TGAATCAGAA AGTGGTCTTG CTGAATTAGA CAGCTCTAAT
541      GAAGATGCAG GGACAAAGAT GAGTGGTGAA AAAATATGA

Quality Control:cDNA sequencing results is available upon request.