Anti-PPM1A Antibody

Cat: 50277-RP02
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Anti-PPM1A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PPM1A Antibody
Validated applications
Species reactivity
Reacts with: Mouse
Mouse PPM1A
Recombinant Mouse PPM1A protein (Catalog#50277-M08E)
Produced in rabbits immunized with purified, recombinant Mouse PPM1A (rM PPM1A; Catalog#50277-M08E; NP_032936.1; Met 1-Trp 382). PPM1A specific IgG was purified by mouse PPM1A affinity chromatography .
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PPM1A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PPM1A Antibody (Rabbit Polyclonal antibody) Images
Mouse PPM1A/PP2CA Immunoprecipitation(IP) 8228

Mouse PPM1A was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

2 µL anti-Mouse PPM1A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse PPM1A rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Mouse PPM1A/PP2CA Western blot (WB) 13659

Anti-PPM1A rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:40 kDa

(We are unsure as to the identity of these extra bands.)

Anti-PPM1A Antibody Alternative Names
Anti-2310003C21Rik Antibody;Anti-2900017D14Rik Antibody;Anti-AI427932 Antibody;Anti-AU017636 Antibody;Anti-MMPa-2 Antibody;Anti-MPPa-1 Antibody
PPM1A Background Information

Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.

Full Name
protein phosphatase, Mg2+/Mn2+ dependent, 1A
  • Lin X, et al. (2006) PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 125(5): 915-28.
  • Marc F, et al. (2003) Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3. Proc Natl Acad. 100 (26): 16006-11.
  • Mann DJ, et al. (1992) Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1130 (1): 100-4.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PPM1A Antibody Rabbit Monoclonal WB 50277-R006
    Anti-PPM1A Antibody Rabbit Polyclonal ELISA 50277-RP01
    Anti-PPM1A Antibody (FITC) Mouse Monoclonal FCM 12984-MM01-F
    Anti-PPM1A Antibody (PE) Mouse Monoclonal FCM 12984-MM01-P
    Anti-PPM1A Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 12984-T50
    Anti-PPM1A Antibody Mouse Monoclonal FCM,ICC/IF,IF 12984-MM01
    Anti-PPM1A Antibody Mouse Monoclonal ELISA 12984-MM07
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