PPM1A / PP2C-alpha Antibody (Antigen Affinity Purified)

Mouse Protein Phosphatase 1A Antibody ( Antigen Affinity Purified )

PPM1A / PP2C-alpha Antibody Product Information

• Immunogen: Recombinant mouse PPM1A protein ( Catalog#50277-M08E )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant mouse PPM1A ( rM PPM1A ; Catalog#50277-M08E ; NP_032936.1 ; Met 1 - Trp 382 ). PPM1A specific IgG was purified by mouse PPM1A affinity chromatography

PPM1A / PP2C-alpha Antibody Usage Guide

• Specificity: Mouse PPM1A / PP2C-alpha
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PPM1A in WB. Using a DAB detection system, the detection limit for Mouse PPM1A is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PPM1A. The detection limit for mouse PPM1A is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PPM1A / PP2C-alpha Antibody Background

Mouse Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha, PP2C-alpha and Protein phosphatase IA and PPM1A, is a member of the PP2C family. PPM1A / PP2C-alpha is a phosphatase which dephosphorylates and inactivates a broad range of substrates, including TGF-beta, MAP kinases, p38 and JNK kinase cascades, and is involved in tumor suppression. PPM1A dephosphorylates and promotes nuclear export of TGFbeta-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFbeta-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFbeta signaling in mammalian cells. Smad-antagonizing activity of PPM1A is observed during Nodal-dependent early embryogenesis in zebrafish. PPM1A / PP2C-alpha plays a critical role in terminating TGFbeta signaling through dephosphorylation of Smad2/3. PPM1A / PP2C-alpha plays a critical role in the regulation of normal placentation by inhibiting trophoblast migration and invasion. PPM1A and to some extent PPM1B are important negative regulators of positive transcription elongation factor b (P-TEFb) function.

References

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4. Zhang,B. et al., 2009, Histochem Cell Biol 132 (2):169-79.
5. Mayya V., et al., 2009, Sci. Signal. 2: RA46-RA46.