PPM1A / PP2C-alpha Protein (His Tag)

Protein phosphatase, Mg²⁺ / Mn²⁺ dependent, 1A Protein Datasheet

PPM1A / PP2C-alpha Protein Product Information

• Synonym: FLJ42306, MGC9201, PP2C-ALPHA, PP2CA, PP2Calpha
• Protein Construction: A DNA sequence encoding the human PPM1A isoform alpha-1 (P35813-1) (Met 1-Trp 382) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: E.coli

PPM1A / PP2C-alpha Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human PPM1A consisting of 392 amino acids and has a calculated molecular mass of 43.8 kDa. It migrates as an approximately 47KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, 10%glycerol, pH 7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PPM1A / PP2C-alpha protein

PPM1A / PP2C-alpha Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PPM1A / PP2C-alpha Protein Description

Mouse Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha, PP2C-alpha and Protein phosphatase IA and PPM1A, is a member of the PP2C family. PPM1A / PP2C-alpha is a phosphatase which dephosphorylates and inactivates a broad range of substrates, including TGF-beta, MAP kinases, p38 and JNK kinase cascades, and is involved in tumor suppression. PPM1A dephosphorylates and promotes nuclear export of TGFbeta-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFbeta-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFbeta signaling in mammalian cells. Smad-antagonizing activity of PPM1A is observed during Nodal-dependent early embryogenesis in zebrafish. PPM1A / PP2C-alpha plays a critical role in terminating TGFbeta signaling through dephosphorylation of Smad2/3. PPM1A / PP2C-alpha plays a critical role in the regulation of normal placentation by inhibiting trophoblast migration and invasion. PPM1A and to some extent PPM1B are important negative regulators of positive transcription elongation factor b (P-TEFb) function.

References

1. Genome Res. 2004, The MGC Project Team.14: 2121-7.
2. Lin X. et al., 2006, Cell. 125 (5): 915-28.
3. Wang Y. et al., 2008, J Biol Chem. 283 (48): 33578-84.
4. Zhang B. et al., 2009, Histochem Cell Biol. 132 (2):169-79.
5. Mayya V. et al., 2009, Sci Signal. 2: RA46.