PPARG / Nr1c3 / PPARgamma Protein (GST Tag)

Peroxisome proliferator-activated receptor gamma Protein Datasheet

PPARG / Nr1c3 / PPARgamma Protein Product Information

• Synonym: CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma
• Protein Construction: A DNA sequence encoding the human PPARG isoform 2 (?P37231-1) (Met 1-Tyr 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

PPARG / Nr1c3 / PPARgamma Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PPARG/GST chimera consists of 742 amino acids and has a calculated molecular mass of 85.4KDa. It migrates as an approximately 85.4 KDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH 7.4, 20%gly, 0.3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PPARG / Nr1c3 / PPARgamma protein

PPARG / Nr1c3 / PPARgamma Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PPARG / Nr1c3 / PPARgamma Protein Description

Peroxisome proliferator-activated receptor gamma, also known as PPARgamma, PPARG, glitazone receptor, or NR1C3, is a typeⅡ nuclear receptor. There are two isoforms of PPARG: PPARG1, which is found in nearly all tissues except nuscle, and PPARG2, which is found in adipose tissue and the intestine. PPARG has functions in modulating fatty acid storage and glucose metabolism. The geens activated by PPARG encode proteins stimulating lipid uptake and adipogenesis by fat cells. PPARG has been implicated in the pathology of numerous diseases, including obesity, diabetes, atherosclerosis and cancer. This receptor has functions in decreaseing the inflammatory response of many cardiovascular cells, especially endothelial cells and activating the PON1 gene that increases synthesis and release of paraoxonase 1 from the liver, reducing atherosclerosis.

References

1. Hamblin M. et al., 2009, Antioxid Redox Signal. 11 (6): 1415-52.
2. Khateeb J. et al., 2010, Atherosclerosis. 208 (1): 119-25.
3. Fajas L. et al., 1997, J Biol Chem. 272 (30): 18779-89.