Human POU5F1 Gene cDNA Clone / ORF Clone

Human POU5F1 cDNA Clone Datasheet

POU5F1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002701.4
• cDNA Size: 1083
• Gene Synonym: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; MGC22487; POU5F1
• Source: Human

POU5F1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens POU class 5 homeobox 1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 943 G/C resulting in the amino acid ala substitution by pro and 21 G/A, 27 C/T,1047 C/T not causing the amino acid variation.

POU5F1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

POU5F1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

POU5F1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGGAC ACCTGGCTTC AGATTTTGCC TTCTCGCCCC CTCCAGGTGG TGGAGGTGAT
61       GGGCCAGGGG GGCCGGAGCC GGGCTGGGTT GATCCTCGGA CCTGGCTAAG CTTCCAAGGC
121      CCTCCTGGAG GGCCAGGAAT CGGGCCGGGG GTTGGGCCAG GCTCTGAGGT GTGGGGGATT
181      CCCCCATGCC CCCCGCCGTA TGAGTTCTGT GGGGGGATGG CGTACTGTGG GCCCCAGGTT
241      GGAGTGGGGC TAGTGCCCCA AGGCGGCTTG GAGACCTCTC AGCCTGAGGG CGAAGCAGGA
301      GTCGGGGTGG AGAGCAACTC CGATGGGGCC TCCCCGGAGC CCTGCACCGT CACCCCTGGT
361      GCCGTGAAGC TGGAGAAGGA GAAGCTGGAG CAAAACCCGG AGGAGTCCCA GGACATCAAA
421      GCTCTGCAGA AAGAACTCGA GCAATTTGCC AAGCTCCTGA AGCAGAAGAG GATCACCCTG
481      GGATATACAC AGGCCGATGT GGGGCTCACC CTGGGGGTTC TATTTGGGAA GGTATTCAGC
541      CAAACGACCA TCTGCCGCTT TGAGGCTCTG CAGCTTAGCT TCAAGAACAT GTGTAAGCTG
601      CGGCCCTTGC TGCAGAAGTG GGTGGAGGAA GCTGACAACA ATGAAAATCT TCAGGAGATA
661      TGCAAAGCAG AAACCCTCGT GCAGGCCCGA AAGAGAAAGC GAACCAGTAT CGAGAACCGA
721      GTGAGAGGCA ACCTGGAGAA TTTGTTCCTG CAGTGCCCGA AACCCACACT GCAGCAGATC
781      AGCCACATCG CCCAGCAGCT TGGGCTCGAG AAGGATGTGG TCCGAGTGTG GTTCTGTAAC
841      CGGCGCCAGA AGGGCAAGCG ATCAAGCAGC GACTATGCAC AACGAGAGGA TTTTGAGGCT
901      GCTGGGTCTC CTTTCTCAGG GGGACCAGTG TCCTTTCCTC TGCCCCCAGG GCCCCATTTT
961      GGTACCCCAG GCTATGGGAG CCCTCACTTC ACTGCACTGT ACTCCTCGGT CCCTTTCCCT
1021     GAGGGGGAAG CCTTTCCCCC TGTCTCTGTC ACCACTCTGG GCTCTCCCAT GCATTCAAAC
1081     TGA

 

Quality Control:cDNA sequencing results is available upon request.