Human POU2AF1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13199-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

POU2AF1 cDNA Clone Datasheet

POU2AF1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_006235.2
cDNA Size:	771
Gene Synonym:	BOB1; OBF1; OCAB; OBF-1;POU2AF1
Source:	Human

POU2AF1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens POU class 2 associating factor 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

POU2AF1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

POU2AF1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

POU2AF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTCTGGC AAAAACCCAC AGCTCCGGAG CAAGCCCCAG CCCCGGCCCG GCCATACCAG
61       GGCGTCCGTG TGAAGGAGCC AGTGAAGGAA CTGCTGAGGA GGAAGCGAGG CCACGCCAGC
121      AGTGGGGCAG CACCTGCACC TACGGCGGTG GTGCTGCCCC ATCAGCCCCT GGCGACCTAC
181      ACCACAGTGG GTCCTTCCTG CCTGGACATG GAAGGTTCTG TGTCTGCAGT GACAGAGGAG
241      GCTGCCCTGT GTGCCGGCTG GCTCTCCCAG CCCACCCCGG CCACCCTGCA GCCCCTGGCC
301      CCATGGACAC CTTACACCGA GTATGTGCCC CATGAAGCTG TCAGCTGCCC CTACTCAGCT
361      GACATGTATG TGCAGCCCGT GTGCCCCAGC TACACGGTGG TGGGGCCCTC CTCAGTGTTG
421      ACCTATGCCT CTCCGCCACT CATCACCAAT GTCACGACAA GAAGCTCCGC CACGCCCGCA
481      GTGGGGCCCC CGCTGGAGGG CCCAGAGCAC CAGGCACCCC TCACCTATTT CCCGTGGCCT
541      CAGCCCCTTT CCACACTACC CACCTCCACC CTGCAGTACC AGCCTCCGGC CCCAGCCCTA
601      CCTGGGCCCC AGTTTGTCCA GCTCCCCATC TCTATCCCAG AGCCAGTCCT TCAGGACATG
661      GAAGACCCCA GAAGAGCCGC CAGCTCGTTG ACCATCGACA AGCTGCTTTT GGAGGAAGAG
721      GATAGCGACG CCTATGCGCT TAACCACACT CTCTCTGTGG AAGGCTTTTA G

Quality Control:cDNA sequencing results is available upon request.