Human POR/CYPOR Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10365-M	Cloning vector ($95) pCMV / hygro ($345) pCMV / hygro - HA ($345) pCMV / hygro - His ($345) pCMV / hygro - Myc ($345) pCMV / hygro - FLAG ($345)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000941.2

cDNA Size:   2043

Synonym:  CPR, CYPOR, P450R, FLJ26468, DKFZp686G04235

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens P450 (cytochrome) oxidoreductase DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the 1009C/T mutation not resulting in amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGATCAACA TGGGAGACTC CCACGTGGAC ACCAGCTCCA CCGTGTCCGA GGCGGTGGCC
61       GAAGAAGTAT CTCTTTTCAG CATGACGGAC ATGATTCTGT TTTCGCTCAT CGTGGGTCTC
121      CTAACCTACT GGTTCCTCTT CAGAAAGAAA AAAGAAGAAG TCCCCGAGTT CACCAAAATT
181      CAGACATTGA CCTCCTCTGT CAGAGAGAGC AGCTTTGTGG AAAAGATGAA GAAAACGGGG
241      AGGAACATCA TCGTGTTCTA CGGCTCCCAG ACGGGGACTG CAGAGGAGTT TGCCAACCGC
301      CTGTCCAAGG ACGCCCACCG CTACGGGATG CGAGGCATGT CAGCGGACCC TGAGGAGTAT
361      GACCTGGCCG ACCTGAGCAG CCTGCCAGAG ATCGACAACG CCCTGGTGGT TTTCTGCATG
421      GCCACCTACG GTGAGGGAGA CCCCACCGAC AATGCCCAGG ACTTCTACGA CTGGCTGCAG
481      GAGACAGACG TGGATCTCTC TGGGGTCAAG TTCGCGGTGT TTGGTCTTGG GAACAAGACC
541      TACGAGCACT TCAATGCCAT GGGCAAGTAC GTGGACAAGC GGCTGGAGCA GCTCGGCGCC
601      CAGCGCATCT TTGAGCTGGG GTTGGGCGAC GACGATGGGA ACTTGGAGGA GGACTTCATC
661      ACCTGGCGAG AGCAGTTCTG GCCGGCCGTG TGTGAACACT TTGGGGTGGA AGCCACTGGC
721      GAGGAGTCCA GCATTCGCCA GTACGAGCTT GTGGTCCACA CCGACATAGA TGCGGCCAAG
781      GTGTACATGG GGGAGATGGG CCGGCTGAAG AGCTACGAGA ACCAGAAGCC CCCCTTTGAT
841      GCCAAGAATC CGTTCCTGGC TGCAGTCACC ACCAACCGGA AGCTGAACCA GGGAACCGAG
901      CGCCACCTCA TGCACCTGGA ATTGGACATC TCGGACTCCA AAATCAGGTA TGAATCTGGG
961      GACCACGTGG CTGTGTACCC AGCCAACGAC TCTGCTCTCG TCAACCAGTT GGGCAAAATC
1021     CTGGGTGCCG ACCTGGACGT CGTCATGTCC CTGAACAACC TGGATGAGGA GTCCAACAAG
1081     AAGCACCCAT TCCCGTGCCC TACGTCCTAC CGCACGGCCC TCACCTACTA CCTGGACATC
1141     ACCAACCCGC CGCGTACCAA CGTGCTGTAC GAGCTGGCGC AGTACGCCTC GGAGCCCTCG
1201     GAGCAGGAGC TGCTGCGCAA GATGGCCTCC TCCTCCGGCG AGGGCAAGGA GCTGTACCTG
1261     AGCTGGGTGG TGGAGGCCCG GAGGCACATC CTGGCCATCC TGCAGGACTG CCCGTCCCTG
1321     CGGCCCCCCA TCGACCACCT GTGTGAGCTG CTGCCGCGCC TGCAGGCCCG CTACTACTCC
1381     ATCGCCTCAT CCTCCAAGGT CCACCCCAAC TCTGTGCACA TCTGTGCGGT GGTTGTGGAG
1441     TACGAGACCA AGGCTGGCCG CATCAACAAG GGCGTGGCCA CCAACTGGCT GCGGGCCAAG
1501     GAGCCTGCCG GGGAGAACGG CGGCCGTGCG CTGGTGCCCA TGTTCGTGCG CAAGTCCCAG
1561     TTCCGCCTGC CCTTCAAGGC CACCACGCCT GTCATCATGG TGGGCCCCGG CACCGGGGTG
1621     GCACCCTTCA TAGGCTTCAT CCAGGAGCGG GCCTGGCTGC GACAGCAGGG CAAGGAGGTG
1681     GGGGAGACGC TGCTGTACTA CGGCTGCCGC CGCTCGGATG AGGACTACCT GTACCGGGAG
1741     GAGCTGGCGC AGTTCCACAG GGACGGTGCG CTCACCCAGC TCAACGTGGC CTTCTCCCGG
1801     GAGCAGTCCC ACAAGGTCTA CGTCCAGCAC CTGCTAAAGC AAGACCGAGA GCACCTGTGG
1861     AAGTTGATCG AAGGCGGTGC CCACATCTAC GTCTGTGGGG ATGCACGGAA CATGGCCAGG
1921     GATGTGCAGA ACACCTTCTA CGACATCGTG GCTGAGCTCG GGGCCATGGA GCACGCGCAG
1981     GCGGTGGACT ACATCAAGAA ACTGATGACC AAGGGCCGCT ACTCCCTGGA CGTGTGGAGC
2041     TAG

Quality Control: cDNA sequencing results is available upon request.