PON3 Protein (His Tag)

Paraoxonase 3 Protein Datasheet

PON3 Protein Product Information

• Synonym: PON3
• Protein Construction: A DNA sequence encoding the human PON3 (Q15166-1) (Met 1- Leu 354) (50 Ser/Asn) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

PON3 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PON3 consists of 373 amino acids and predicts a molecular mass of 42 kDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 1mM CaCL2, 10%gly, 0.1% DDM
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PON3 protein

PON3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PON3 Protein Description

Serum paraoxonase / lactonase 3, also known as PON3, is a secreted protein which belongs to the paraoxonase family. The paraoxonase (PON) gene family in humans has three members, PON1, PON2, and PON3, which are known to be involved in oxidative stress-associated processes such as dyslipidemia, diabetes and coronary heart disease. Although PON3 is the least studied paraoxonase, findings have shown that it can significantly reduce atherosclerotic lesion formation and obesity in PON3 transgenic mice. Enzymatic characterization of the purified PONs has revealed them to be lactonases/lactonizing enzymes, with some overlapping substrates, but also to have distinctive substrate specificities. All three PONs metabolized very efficiently 5-hydroxy-eicosatetraenoic acid 1,5-lactone and 4-hydroxy-docosahexaenoic acid, which are products of both enzymatic and nonenzymatic oxidation of arachidonic acid and docosahexaenoic acid, respectively, and may represent the PONs' endogenous substrates. PON3 has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. It rapidly hydrolyzes lactones such as statin prodrugs. PON3 hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents. PON3 is also involved in obesity-related disorders.

References

1. Draganov DI. et al., 2005, J Lipid Res. 46: 1239-47.
2. Pla A. et al., 2007, Chem Biol Interact. 167 (1): 63-70.
3. Sanghera DK. et al., 2008, Ann Hum Genet. 72 (1): 72-81.
4. Labrecque B. et al., 2009, Gene. 443 (1-2):110-20.