Human POMC Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13213-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

POMC cDNA Clone Datasheet

POMC cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001035256.1
cDNA Size:	804
Gene Synonym:	LPH; MSH; NPP; POC; ACTH; CLIP; POMC
Source:	Human

POMC cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens proopiomelanocortin DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

POMC cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

POMC cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

POMC cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCGAGAT CGTGCTGCAG CCGCTCGGGG GCCCTGTTGC TGGCCTTGCT GCTTCAGGCC
61       TCCATGGAAG TGCGTGGCTG GTGCCTGGAG AGCAGCCAGT GTCAGGACCT CACCACGGAA
121      AGCAACCTGC TGGAGTGCAT CCGGGCCTGC AAGCCCGACC TCTCGGCCGA GACTCCCATG
181      TTCCCGGGAA ATGGCGACGA GCAGCCTCTG ACCGAGAACC CCCGGAAGTA CGTCATGGGC
241      CACTTCCGCT GGGACCGATT CGGCCGCCGC AACAGCAGCA GCAGCGGCAG CAGCGGCGCA
301      GGGCAGAAGC GCGAGGACGT CTCAGCGGGC GAAGACTGCG GCCCGCTGCC TGAGGGCGGC
361      CCCGAGCCCC GCAGCGATGG TGCCAAGCCG GGCCCGCGCG AGGGCAAGCG CTCCTACTCC
421      ATGGAGCACT TCCGCTGGGG CAAGCCGGTG GGCAAGAAGC GGCGCCCAGT GAAGGTGTAC
481      CCTAACGGCG CCGAGGACGA GTCGGCCGAG GCCTTCCCCC TGGAGTTCAA GAGGGAGCTG
541      ACTGGCCAGC GACTCCGGGA GGGAGATGGC CCCGACGGCC CTGCCGATGA CGGCGCAGGG
601      GCCCAGGCCG ACCTGGAGCA CAGCCTGCTG GTGGCGGCCG AGAAGAAGGA CGAGGGCCCC
661      TACAGGATGG AGCACTTCCG CTGGGGCAGC CCGCCCAAGG ACAAGCGCTA CGGCGGTTTC
721      ATGACCTCCG AGAAGAGCCA GACGCCCCTG GTGACGCTGT TCAAAAACGC CATCATCAAG
781      AACGCCTACA AGAAGGGCGA GTGA

Quality Control:cDNA sequencing results is available upon request.