Human POFUT1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12194-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

POFUT1 cDNA Clone Datasheet

POFUT1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC000582
cDNA Size:	585
Gene Synonym:	FUT12; O-FUT; O-Fuc-T; O-FucT-1;POFUT1
Source:	Human

POFUT1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protein O-fucosyltransferase 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

POFUT1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

POFUT1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

POFUT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGCCG CCGCGTGGGC ACGGCCGCTG AGCGTGTCTT TCCTGCTGCT GCTTCTGCCG
61       CTCCCGGGGA TGCCTGCGGG CTCCTGGGAC CCGGCCGGTT ACCTGCTCTA CTGCCCCTGC
121      ATGGGGCGCT TTGGGAACCA GGCCGATCAC TTCTTGGGCT CTCTGGCATT TGCAAAGCTG
181      CTAAACCGTA CCTTGGCTGT CCCTCCTTGG ATTGAGTACC AGCATCACAA GCCTCCTTTC
241      ACCAACCTCC ATGTGTCCTA CCAGAAGTAC TTCAAGCTGG AGCCCCTCCA GGCTTACCAT
301      CGGGTCATCA GCTTGGAGGA TTTCATGGAG AAGCTGGCAC CCACCCACTG GCCCCCTGAG
361      AAGCGGGTGG CATACTGCTT TGAGGTGGCA GCCCAGCGAA GCCCAGATAA GAAGACGTGC
421      CCCATGAAGG AAGGAAACCC CTTTGGCCCA TTCTGGGATC AGTTTCATGT GAGTTTCAAC
481      AAGTCGGAGC TTTTTACAGG CATTTCCTTC AGTGCTTCCT ACAGAGAACA ATGGAGCCAG
541      AGGCGTGAGA ATCACTCCTG TGTTACCTTA CTCTTCCCAA GGTGA

Quality Control:cDNA sequencing results is available upon request.