Human PNMT Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13873-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PNMT cDNA Clone Datasheet

PNMT cDNA Clone Product Information

Gene Bank Ref.ID:	BC037246
cDNA Size:	849
Gene Synonym:	MGC34570, PENT, PNMTase,PNMT
Source:	Human

PNMT cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens phenylethanolamine N-methyltransferase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PNMT cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PNMT cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PNMT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCGGCG CAGACCGTAG CCCCAATGCG GGCGCAGCCC CTGACTCGGC CCCGGGCCAG
61       GCGGCGGTGG CTTCGGCCTA CCAGCGCTTC GAGCCGCGCG CCTACCTCCG CAACAACTAC
121      GCGCCCCCTC GCGGGGACCT GTGCAACCCG AACGGCGTCG GGCCGTGGAA GCTGCGCTGC
181      TTGGCGCAGA CCTTCGCCAC CGGTGAAGTG TCCGGACGCA CCCTCATCGA CATTGGTTCA
241      GGCCCCACCG TGTACCAGCT GCTCAGTGCC TGCAGCCACT TTGAGGACAT CACCATGACA
301      GATTTCCTGG AGGTCAACCG CCAGGAGCTG GGGCGCTGGC TGCAGGAGGA GCCGGGGGCC
361      TTCAACTGGA GCATGTACAG CCAACATGCC TGCCTCATTG AGGGCAAGGG GGAATGCTGG
421      CAGGATAAGG AGCGCCAGCT GCGAGCCAGG GTGAAACGGG TCCTGCCCAT CGACGTGCAC
481      CAGCCCCAGC CCCTGGGTGC TGGGAGCCCA GCTCCCCTGC CTGCTGACGC CCTGGTCTCT
541      GCCTTCTGCT TGGAGGCTGT GAGCCCAGAT CTTGCCAGCT TTCAGCGGGC CCTGGACCAC
601      ATCACCACGC TGCTGAGGCC TGGGGGGCAC CTCCTCCTCA TCGGGGCCCT GGAGGAGTCG
661      TGGTACCTGG CTGGGGAGGC CAGGCTGACG GTGGTGCCAG TGTCTGAGGA GGAGGTGAGG
721      GAGGCCCTGG TGCGTAGTGG CTACAAGGTC CGGGACCTCC GCACCTATAT CATGCCTGCC
781      CACCTTCAGA CAGGCGTAGA TGATGTCAAG GGCGTCTTCT TCGCCTGGGC TCAGAAGGTT
841      GGGCTGTGA

Quality Control:cDNA sequencing results is available upon request.