|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of pancreatic lipase-related protein 2 DNA.|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human PNLIPRP2 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13687-G-F|
|Human PNLIPRP2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13687-G-H|
|Human PNLIPRP2 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13687-G-M|
|Human PNLIPRP2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13687-G-N|
|Human PNLIPRP2 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13687-G-Y|
|Product name||Product name|
Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. It can hydrolyze both phospholipids and galactolipids. Galactolipase acts preferentially on monoglycerides, phospholipids and galactolipids. It also hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase.