PNLIP Protein (His Tag)

Pancreatic lipase Protein Datasheet

PNLIP Protein Product Information

• Synonym: PNLIP
• Protein Construction: A DNA sequence encoding the human PNLIP (P16233) (Met1-Cys465) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

PNLIP Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 17
• Molecular Mass: The recombinant human PNLIP consists of 460 amino acids and predicts a molecular mass of 51 KDa. It migrates as an approximately 51 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PNLIP protein

PNLIP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PNLIP Protein Description

PNLIP is an enzyme which belongs to the lipase family. It is essential for the efficient digestion of dietary fats. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively.

References

1. Hegele RA. et al., 2001, J Hum Genet. 46 (6): 320-4.
2. Thomas A. et al., 2005, J Biol Chem. 280 (48): 40074-83.
3. Colin DY. et al., 2008, Biochem Biophys Res Commun. 370 (3): 394-8.