PMP2 / FABP8 Antibody (Antigen Affinity Purified)

Myelin P2 Protein Antibody ( Antigen Affinity Purified )

PMP2 / FABP8 Antibody Product Information

• Immunogen: Recombinant human PMP2 protein ( Catalog#11426-H07E )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human PMP2 ( rh PMP2 ; Catalog#11426-H07E ; NP_002668.1 ; Ser 2 - Val 132 ). PMP2 specific IgG was purified by human PMP2 affinity chromatography

PMP2 / FABP8 Antibody Usage Guide

• Specificity: Human PMP2 / FABP8
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human PMP2 in WB. Using a DAB detection system, the detection limit for human PMP2 is approximately 0.5 ng/lane under non-reducing conditions reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human PMP2. The detection limit for human PMP2 is approximately 0.039 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PMP2 / FABP8 Antibody Background

Myelin P2 protein, also known as PMP2, is a cytosolic protein found primarily in peripheral nerves. It Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. PMP2 is a small, basic, and cytoplasmic lipid binding protein of peripheral myelin. It is similar in amino acid sequence and tertiary structure to fatty acid binding proteins found in the liver, adipocytes, and intestine, its expression is limited to the nervous system. PMP2 is detected only in myelin-producing cells of the central and peripheral nervous systems, the oligodendrocytes and Schwann cells, respectively. PMP2 may play a role in lipid transport protein in Schwann cells. It forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.

References

1. Hayasaka, K. et al., 1993, Genomics. 18 (2): 244-8.
2. Polverini, E. et al., 2006, J Struct Biol. 153 (3): 253-63.
3. Gould,R.M. et al., 2008, Neuron Glia Biol. 4 (2):137-52.