Mouse PLK4/SAK Gene cDNA Clone / ORF Clone

PLK4 cDNA Clone Datasheet

PLK4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011495.2
• cDNA Size: 2778
• Gene Synonym: Sak; Stk18; AI385771; 1700028H20; Plk4
• Source: Mouse

PLK4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus polo-like kinase 4 (Drosophila) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PLK4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PLK4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PLK4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCGT GCATCGGGGA GAGGATCGAG GACTTTAAGG TTGGAAATCT ACTCGGTAAA
61       GGATCATTTG CTGGTGTCTA CAGAGCTGAG TCCATACACA CTGGTTTGGA AGTTGCAATC
121      AAAATGATAG ATAAGAAAGC CATGTACAAA GCTGGAATGG TACAGAGAGT CCAAAATGAG
181      GTGAAAATAC ATTGCCAGTT GAAACACCCC TCTGTCTTGG AGCTCTATAA TTACTTTGAA
241      GATAACAATT ATGTCTACCT GGTATTGGAA ATGTGCCACA ATGGAGAAAT GAACAGATAT
301      CTGAAGAACA GAATGAAGCC TTTCTCAGAA AGGGAAGCTA GGCACTTCAT GCACCAGATT
361      ATCACAGGAA TGTTATATCT TCATTCTCAT GGCATATTGC ACCGGGACCT CACACTCTCT
421      AACATCTTAC TTACGCGGAA TATGAACATA AAAATTGCTG ACTTTGGACT AGCAACGCAG
481      TTGAATATGC CACATGAAAA GCACTATACA CTCTGTGGGA CTCCTAATTA TATTTCACCA
541      GAAATTGCAA CTCGAAGTGC ACATGGACTT GAATCTGATA TTTGGTCATT GGGCTGTATG
601      TTTTATACGT TACTTATTGG AAGACCACCT TTTGACACTG ACACAGTCAA GAACACATTG
661      AACAAAGTAG TCCTGGCAGA TTATGAAATG CCAGCCTTTT TGTCACGAGA GGCCCAGGAC
721      CTTATCCACC AGTTACTTCG TAGAAACCCT GCAGATCGGT TAAGTCTGTC TTCTGTGTTG
781      GACCATCCTT TCATGTCACG AAATCCTTCA CCAAAGAGTA AAGACGTAGG GACTGTAGAG
841      GACTCAATGG ATAGTGGGCA TGCTACACTT TCCACAACAA TTACAGCCTC TTCTGGTACC
901      AGTTTGAGTG GCAGCCTACT TGACAGAAGA CTTTTGGTTG GTCAACCACT TCCAAATAAA
961      ATTACTGTAT TTCAAAAAAA TAAAAATTCA AGTGACTTTT CTTCAGGAGA TGGAAGTAAT
1021     TTTTGTACTC AATGGGGAAA TCCAGAACAA GAAGCTAATA GTAGGGGACG GGGGAGAGTG
1081     ATTGAAGATG CAGAAGAGAG GCCGCATTCT CGATACCTGC GCAGAGCTCA TTCCTCTGAT
1141     AGAGCCAGCC CCTCTAATCA GTCTCGAGCA AAAACATACT CAGTAGAACG TTGTCACTCA
1201     GTAGAAATGC TTTCAAAGCC TAGAAGATCA CTGGATGAAA ATCAACACAG TTCCAATCAT
1261     CATTGTCTAG GAAAAACTCC TTTTCCATTT GCAGACCAGA CACCTCAGAT GGAAATGGTA
1321     CAGCAGTGGT TTGGGAATCT GCAAATGAAT GCTCATTTAG GAGAAACTAA TGAGCACCAC
1381     ACCGTTAGCC CAAACAGAGA TTTCCAGGAC TATCCAGATT TGCAGGACAC GTTACGAAAC
1441     GCTTGGACTG ACACGAGAGC CAGCAAGAAT GCTGATACTT CTGCCAATGT TCATGCTGTA
1501     AAGCAGCTGA GTGCCATGAA ATACATGAGT GCACATCACC ATAAGCCTGA GGTCATGCCA
1561     CAGGAGCCGG GCCTACATCC TCATTCTGAA CAAAGCAAGA ATAGAAGTAT GGAGTCGACA
1621     CTGGGTTACC AGAAACCTAC CTTAAGAAGT ATTACATCTC CTCTGATTGC TCACAGATTA
1681     AAGCCAATCA GACAGAAAAC CAAAAAGGCT GTGGTGAGCA TCCTTGATTC AGAGGAGGTG
1741     TGTGTGGAGC TTCTGAGAGA GTGTGCGTCT GAAGGATATG TGAAAGAAGT GCTTCAGATA
1801     TCGAGTGATG GGACTATGAT CACTGTTTAT TACCCGAACG ATGGAAGAGG CTTTCCTCTT
1861     GCTGACAGAC CTCCCTTGCC TACTGACAAC ATCAGTAGGT ACAGCTTTGA CAATCTACCA
1921     GAAAAATACT GGCGGAAATA TCAGTATGCT TCCAGATTCA TTCAGCTAGT AAGATCTAAA
1981     ACTCCCAAAA TCACTTATTT TACAAGATAT GCTAAATGTA TTTTGATGGA AAATTCTCCT
2041     GGTGCTGATT TCGAAGTTTG GTTTTATGAT GGAGCCAAAA TACATAAAAC TGAAAATTTA
2101     ATTCACATAA TTGAGAAAAC AGGGATATCT TATAATTTAA AAAATGAAAA TGAAGTTACC
2161     AGCCTGAAAG AGGAAGTAAA AGTATATATG GACCATGCTA ATGAGGGTCA CCGTATTTGC
2221     TTGTCACTGG AATCTGTAAT CTCTGAGGAG GAAAAGAGAA GCAGGGGTTC TTCATTCTTC
2281     CCTATAATCG TAGGAAGAAA ACCTGGTAAT ACTAGTTCAC CTAAAGCCTT ATCAGCTCCT
2341     CCTGTGGACC CAAGCTGCTG TAAGGGAGAG CAGGCGTCAG CAAGCAGACT GAGCGTGAAT
2401     AGTGCCGCTT TCCCCACACA GTCCCCAGGA CTCAGTCCTT CCACTGTGAC AGTTGAAGGA
2461     CTTGGCCACA CAGCGACTGC CACAGGAACA GGCGTCTCTT CAAGTCTTCC TAAATCTGCA
2521     CAGCTTTTGA AATCTGTTTT TGTGAAAAAT GTTGGTTGGG CTACACAGCT AACTAGCGGA
2581     GCTGTGTGGG TTCAGTTTAA TGATGGGTCA CAGTTGGTTG TCCAGGCAGG AGTATCTTCC
2641     ATCAGTTACA CATCACCAGA TGGTCAGACA ACTAGGTATG GAGAAAATGA AAAATTACCT
2701     GAATACATCA AACAGAAATT ACAGTGTCTT TCTTCCATCC TTCTGATGTT TTCTAATCCA
2761     ACTCCTAATT TTCAGTAA

 

Quality Control:cDNA sequencing results is available upon request.