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Human PLD1 Gene cDNA Clone (full-length ORF Clone)
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Human PLD1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG13850-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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PLD1 cDNA Clone Datasheet

 

PLD1 cDNA Clone Product Information

Gene Bank Ref.ID: BC068976

cDNA Size:

3225

Gene Synonym:

PLD1

Source: Human

PLD1 cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens phospholipase D1, phosphatidylcholine-specific DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PLD1 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PLD1 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PLD1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCACTGA AAAACGAGCC ACGGGTAAAT ACCTCTGCAC TGCAGAAAAT TGCTGCTGAC
61       ATGAGTAATA TCATAGAAAA TCTGGACACG CGGGAACTCC ACTTTGAGGG AGAGGAGGTA
121      GACTACGACG TGTCTCCCAG CGATCCCAAG ATACAAGAAG TGTATATCCC TTTCTCTGCT
181      ATTTATAACA CTCAAGGATT TAAGGAGCCT AATATACAGA CGTATCTCTC CGGCTGTCCA
241      ATAAAAGCAC AAGTTCTGGA AGTGGAACGC TTCACATCTA CAACAAGGGT ACCAAGTATT
301      AATCTTTACA CTATTGAATT AACACATGGG GAATTTAAAT GGCAAGTTAA GAGGAAATTC
361      AAGCATTTTC AAGAATTTCA CAGAGAGCTG CTCAAGTACA AAGCCTTTAT CCGCATCCCC
421      ATTCCCACTA GAAGACACAC GTTTAGGAGG CAAAACGTCA GAGAGGAGCC TCGAGAGATG
481      CCCAGTTTGC CCCGTTCATC TGAAAACATG ATAAGAGAAG AACAATTCCT TGGTAGAAGA
541      AAACAACTGG AAGATTACTT GACAAAGATA CTAAAAATGC CCATGTATAG AAACTATCAT
601      GCCACAACAG AGTTTCTTGA TATAAGCCAG CTGTCTTTCA TCCATGATTT GGGACCAAAG
661      GGCATAGAAG GTATGATAAT GAAAAGATCT GGAGGACACA GAATACCAGG CTTGAATTGC
721      TGTGGTCAGG GAAGAGCCTG CTACAGATGG TCAAAAAGAT GGTTAATAGT GAAAGATTCC
781      TTTTTATTGT ATATGAAACC AGACAGCGGT GCCATTGCCT TCGTCCTGCT GGTAGACAAA
841      GAATTCAAAA TTAAGGTGGG GAAGAAGGAG ACAGAAACGA AATATGGAAT CCGAATTGAT
901      AATCTTTCAA GGACACTTAT TTTAAAATGC AACAGCTATA GACATGCTCG GTGGTGGGGA
961      GGGGCTATAG AAGAATTCAT CCAGAAACAT GGCACCAACT TTCTCAAAGA TCATCGATTT
1021     GGGTCATATG CTGCTATCCA AGAGAATGCT TTAGCTAAAT GGTATGTTAA TGCCAAAGGA
1081     TATTTTGAAG ATGTGGCAAA TGCAATGGAA GAGGCAAATG AAGAGATTTT TATCACAGAC
1141     TGGTGGCTGA GTCCAGAAAT CTTCCTGAAA CGCCCAGTGG TTGAGGGAAA TCGTTGGAGG
1201     TTGGACTGCA TTCTTAAACG AAAAGCACAA CAAGGAGTGA GGATCTTCAT AATGCTCTAC
1261     AAAGAGGTGG AACTCGCTCT TGGCATCAAT AGTGAATACA CCAAGAGGAC TTTGATGCGT
1321     CTACATCCCA ACATAAAGGT GATGAGACAC CCGGATCATG TGTCATCCAC CGTCTATTTG
1381     TGGGCTCACC ATGAGAAGCT TGTCATCATT GACCAATCGG TGGCCTTTGT GGGAGGGATT
1441     GACCTGGCCT ATGGAAGGTG GGACGACAAT GAGCACAGAC TCACAGACGT GGGCAGTGTG
1501     AAGCGGGTCA CTTCAGGACC GTCTCTGGGT TCCCTCCCAC CTGCCGCAAT GGAGTCTATG
1561     GAATCCTTAA GACTCAAAGA TAAAAATGAG CCTGTTCAAA ACCTACCCAT CCAGAAGAGT
1621     ATTGATGATG TGGATTCAAA ACTGAAAGGA ATAGGAAAGC CAAGAAAGTT CTCCAAATTT
1681     AGTCTCTACA AGCAGCTCCA CAGGCACCAC CTGCACGACG CAGATAGCAT CAGCAGCATT
1741     GACAGCACCT CCAGTTATTT TAATCACTAT AGAAGTCATC ACAATTTAAT CCATGGTTTA
1801     AAACCCCACT TCAAACTCTT TCACCCGTCC AGTGAGTCTG AGCAAGGACT CACTAGACCT
1861     CATGCTGATA CCGGGTCCAT CCGTAGTTTA CAGACAGGTG TGGGAGAGCT GCATGGGGAA
1921     ACCAGATTCT GGCATGGAAA GGACTACTGC AATTTCGTCT TCAAAGACTG GGTTCAACTT
1981     GATAAACCTT TTGCTGATTT CATTGACAGG TACTCCACGC CCCGGATGCC CTGGCATGAC
2041     ATTGCCTCTG CAGTCCACGG GAAGGCGGCT CGTGATGTGG CACGTCACTT CATCCAGCGC
2101     TGGAACTTCA CAAAAATTAT GAAATCAAAA TATCGGTCCC TTTCTTATCC TTTTCTGCTT
2161     CCAAAGTCTC AAACAACAGC CCATGAGTTG AGATATCAAG TGCCTGGGTC TGTCCATGCT
2221     AACGTACAGT TGCTCCGCTC TGCTGCTGAT TGGTCTGCTG GTATAAAGTA CCATGAAGAG
2281     TCCATCCACG CCGCTTACGT CCATGTGATA GAGAACAGCA GGCACTATAT CTATATCGAA
2341     AACCAGTTTT TCATAAGCTG TGCTGATGAC AAAGTTGTGT TCAACAAGAT AGGCGATGCC
2401     ATTGCCCAGA GGATCCTGAA AGCTCACAGG GAAAACCAGA AATACCGGGT ATATGTCGTG
2461     ATACCACTTC TGCCAGGGTT CGAAGGAGAC ATTTCAACCG GCGGAGGAAA TGCTCTACAG
2521     GCAATCATGC ACTTCAACTA CAGAACCATG TGCAGAGGAG AAAATTCCAT CCTTGGACAG
2581     TTAAAAGCAG AGCTTGGTAA TCAGTGGATA AATTACATAT CATTCTGTGG TCTTAGAACA
2641     CATGCAGAGC TCGAAGGAAA CCTAGTAACT GAGCTTATCT ATGTCCACAG CAAGTTGTTA
2701     ATTGCTGATG ATAACACTGT TATTATTGGC TCTGCCAACA TAAATGACCG CAGCATGCTG
2761     GGAAAGCGTG ACAGTGAAAT GGCTGTCATT GTGCAAGATA CAGAGACTGT TCCTTCAGTA
2821     ATGGATGGAA AAGAGTACCA AGCTGGCCGG TTTGCCCGAG GACTTCGGCT ACAGTGCTTT
2881     AGGGTTGTCC TTGGCTATCT TGATGACCCA AGTGAGGACA TTCAGGATCC AGTGAGTGAC
2941     AAATTCTTCA AGGAGGTGTG GGTTTCAACA GCAGCTCGAA ATGCTACAAT TTATGACAAG
3001     GTTTTCCGGT GCCTTCCCAA TGATGAAGTA CACAATTTAA TTCAGCTGAG AGACTTTATA
3061     AACAAGCCCG TATTAGCTAA GGAAGATCCC ATTCGAGCTG AGGAGGAACT GAAGAAGATC
3121     CGTGGATTTT TGGTGCAATT CCCCTTTTAT TTCTTGTCTG AAGAAAGCCT ACTGCCTTCT
3181     GTTGGGACCA AAGAGGCCAT AGTGCCCATG GAGGTTTGGA CTTAA

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"