Human PLA2G4F Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13671-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PLA2G4F cDNA Clone Datasheet

PLA2G4F cDNA Clone Product Information

Gene Bank Ref.ID:	NM_213600.3
cDNA Size:	2550
Gene Synonym:	PLA2G4FZ; PLA2G4F
Source:	Human

PLA2G4F cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens phospholipase A2, group IVF DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 2218 A/G resulting in the amino acid Met substitution by Val.

PLA2G4F cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PLA2G4F cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PLA2G4F cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTTGGG CACTCTGGCC AAGGTGGCTG GCAGACAAGA TGCTGCCCCT CCTGGGGGCA
61       GTGCTGCTTC AGAAGAGAGA GAAGAGGGGC CCTCTGTGGA GGCACTGGCG GCGGGAAACC
121      TACCCATACT ATGACCTCCA GGTGAAGGTG CTGAGGGCCA CAAACATCCG GGGCACAGAC
181      CTGCTGTCCA AAGCCGACTG CTATGTGCAA CTGTGGCTGC CCACGGCGTC CCCAAGCCCT
241      GCCCAGACTA GGATAGTGGC CAACTGCAGT GACCCCGAGT GGAATGAGAC CTTCCACTAC
301      CAGATCCATG GTGCTGTGAA GAACGTCCTG GAGCTCACCC TCTATGACAA GGACATCCTG
361      GGCAGCGACC AGCTCTCTCT GCTCCTGTTT GACCTGAGAA GCCTCAAGTG TGGCCAACCT
421      CACAAACACA CCTTCCCACT CAACCACCAG GATTCACAAG AGCTGCAGGT GGAATTTGTT
481      CTGGAGAAGA GCCAGGTGCC TGCATCTGAA GTCATCACCA ACGGGGTTCT GGTGGCTCAC
541      CCCTGTCTGA GAATCCAGGG CACGCTCCGG GGAGATGGGA CAGCCCCACG GGAAGAGTAC
601      GGCTCTAGGC AGCTCCAGCT GGCAGTGCCT GGAGCCTACG AGAAGCCACA GCTCTTGCCC
661      CTGCAGCCTC CCACAGAGCC AGGCCTCCCA CCCACCTTTA CCTTCCACGT GAACCCAGTG
721      CTGAGCTCCA GGCTACACGT GGAGCTGATG GAGCTGCTGG CAGCTGTGCA GAGTGGCCCC
781      AGCGCAGAGT TGGAGGCTCA GACCAGCAAG CTGGGCGAGG GGGGCATCCT GCTCTCCTCT
841      CTGCCCCTAG GCCAGGAGGA ACAGTGTTCT GTGGCCCTGG GGGAGGGCCA GGAGGTGGCT
901      CTGAGCATGA AGGTGGAAAT GAGCTCCGGG GACCTAGACC TACGCCTTGG CTTTGACCTC
961      TCTGACGGGG AGCAGGAGTT TCTGGACAGG AGGAAGCAGG TCGTGTCCAA GGCCCTGCAG
1021     CAAGTGCTGG GATTGAGTGA GGCTCTGGAC AGTGGCCAGG TGCCTGTAGT GGCTGTGTTG
1081     GGTTCCGGGG GTGGAACCCG AGCCATGTCT TCTCTGTACG GCAGCCTGGC AGGGTTGCAG
1141     GAGCTCGGCC TTCTAGACAC TGTGACCTAC CTGAGTGGGG TCTCTGGGTC TACCTGGTGC
1201     ATCTCCACAC TCTACAGGGA CCCAGCCTGG TCCCAGGTGG CCTTGCAGGG CCCCATTGAG
1261     CGTGCCCAGG TTCACGTCTG CAGCAGTAAG ATGGGAGCTT TGTCCACGGA GCGGCTACAG
1321     TACTACACTC AGGAACTGGG GGTCCGGGAG CGCAGTGGCC ACAGCGTGTC CCTCATCGAC
1381     CTCTGGGGCC TCCTTGTTGA GTATCTCCTG TACCAGGAGG AGAACCCTGC CAAGCTGTCT
1441     GACCAACAGG AGGCGGTCCG CCAGGGTCAG AACCCTTACC CCATTTACAC CAGTGTCAAC
1501     GTCCGCACCA ACTTGAGTGG GGAAGATTTT GCAGAGTGGT GCGAGTTCAC GCCCTATGAG
1561     GTTGGCTTCC CCAAGTACGG GGCTTATGTT CCCACCGAGC TCTTCGGCTC AGAACTCTTC
1621     ATGGGACGAT TGCTGCAGCT CCAGCCTGAA CCCCGGATCT GTTACCTGCA AGGTATGTGG
1681     GGCAGCGCCT TTGCCACCAG CCTGGATGAG ATCTTCCTAA AGACCGCCGG CTCGGGCCTC
1741     AGCTTCCTGG AGTGGTACAG AGGCAGTGTG AATATCACAG ACGACTGCCA GAAGCCTCAG
1801     CTGCACAACC CCTCGAGGCT GCGAACGAGG CTCCTCACCC CACAGGGGCC CTTCTCCCAG
1861     GCTGTGCTGG ACATATTCAC CTCCCGCTTC ACTTCCGCCC AGAGCTTTAA CTTCACCCGG
1921     GGTCTCTGCT TGCACAAGGA CTATGTGGCT GGCAGGGAGT TCGTGGCCTG GAAAGACACA
1981     CACCCGGACG CCTTCCCCAA CCAGCTCACC CCCATGCGGG ACTGCCTGTA CCTGGTGGAC
2041     GGAGGCTTTG CCATCAACTC TCCGTTCCCA CTGGCTCTGC TGCCTCAGAG AGCAGTGGAC
2101     CTCATTCTGT CCTTTGACTA TTCCTTGGAA GCCCCTTTTG AGGTCTTGAA GATGACAGAG
2161     AAGTACTGCC TGGACCGAGG AATCCCCTTC CCTGGCATCG AGGTGGGCCC TGAGGACATG
2221     GAGGAGGCCC GTGAGTGCTA TCTGTTTGCC AAGGCTGAGG ACCCCCGCTC CCCCATTGTG
2281     CTGCACTTCC CCCTGGTTAA CCGTACCTTC CGCACACACC TGGCCCCAGG TGTGGAGCGA
2341     CAAACAGCTG AGGAGAAGGC CTTTGGGGAC TTTGTCATCA ACAGGCCAGA CACCCCCTAT
2401     GGCATGATGA ACTTCACCTA TGAGCCCCAG GACTTTTATC GGCTGGTGGC CCTCAGTCGA
2461     TACAACGTCC TGAACAACGT GGAGACCTTG AAGTGCGCCC TCCAGCTGGC TCTGGACCGG
2521     CACCAGGCTC GGGAGAGGGC AGGGGCCTGA

Quality Control:cDNA sequencing results is available upon request.