Human PLA2G4A Gene cDNA Clone / ORF Clone

Human PLA2G4A cDNA Clone Datasheet

PLA2G4A cDNA Clone Product Information

• Gene Bank Ref.ID: NM_024420.2
• cDNA Size: 2250
• Gene Synonym: PLA2G4; MGC126350; cPLA2-alpha; PLA2G4A
• Source: Human

PLA2G4A cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens phospholipase A2, group IVA (cytosolic, calcium-dependent) DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1952 A/G resulting in the amino acid Lys substitution by Arg.

PLA2G4A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PLA2G4A cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PLA2G4A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCATTTA TAGATCCTTA CCAGCACATT ATAGTGGAGC ACCAGTATTC CCACAAGTTT
61       ACGGTAGTGG TGTTACGTGC CACCAAAGTG ACAAAGGGGG CCTTTGGTGA CATGCTTGAT
121      ACTCCAGATC CCTATGTGGA ACTTTTTATC TCTACAACCC CTGACAGCAG GAAGAGAACA
181      AGACATTTCA ATAATGACAT AAACCCTGTG TGGAATGAGA CCTTTGAATT TATTTTGGAT
241      CCTAATCAGG AAAATGTTTT GGAGATTACG TTAATGGATG CCAATTATGT CATGGATGAA
301      ACTCTAGGGA CAGCAACATT TACTGTATCT TCTATGAAGG TGGGAGAAAA GAAAGAAGTT
361      CCTTTTATTT TCAACCAAGT CACTGAAATG GTTCTAGAAA TGTCTCTTGA AGTTTGCTCA
421      TGCCCAGACC TACGATTTAG TATGGCTCTG TGTGATCAGG AGAAGACTTT CAGACAACAG
481      AGAAAAGAAC ACATAAGGGA GAGCATGAAG AAACTCTTGG GTCCAAAGAA TAGTGAAGGA
541      TTGCATTCTG CACGTGATGT GCCTGTGGTA GCCATATTGG GTTCAGGTGG GGGTTTCCGA
601      GCCATGGTGG GATTCTCTGG TGTGATGAAG GCATTATACG AATCAGGAAT TCTGGATTGT
661      GCTACCTACG TTGCTGGTCT TTCTGGCTCC ACCTGGTATA TGTCAACCTT GTATTCTCAC
721      CCTGATTTTC CAGAGAAAGG GCCAGAGGAG ATTAATGAAG AACTAATGAA AAATGTTAGC
781      CACAATCCCC TTTTACTTCT CACACCACAG AAAGTTAAAA GATATGTTGA GTCTTTATGG
841      AAGAAGAAAA GCTCTGGACA ACCTGTCACC TTTACTGATA TCTTTGGGAT GTTAATAGGA
901      GAAACACTAA TTCATAATAG AATGAATACT ACTCTGAGCA GTTTGAAGGA AAAAGTTAAT
961      ACTGCACAAT GCCCTTTACC TCTTTTCACC TGTCTTCATG TCAAACCTGA CGTTTCAGAG
1021     CTGATGTTTG CAGATTGGGT TGAATTTAGT CCATACGAAA TTGGCATGGC TAAATATGGT
1081     ACTTTTATGG CTCCCGACTT ATTTGGAAGC AAATTTTTTA TGGGAACAGT CGTTAAGAAG
1141     TATGAAGAAA ACCCCTTGCA TTTCTTAATG GGTGTCTGGG GCAGTGCCTT TTCCATATTG
1201     TTCAACAGAG TTTTGGGCGT TTCTGGTTCA CAAAGCAGAG GCTCCACAAT GGAGGAAGAA
1261     TTAGAAAATA TTACCACAAA GCATATTGTG AGTAATGATA GCTCGGACAG TGATGATGAA
1321     TCACACGAAC CCAAAGGCAC TGAAAATGAA GATGCTGGAA GTGACTATCA AAGTGATAAT
1381     CAAGCAAGTT GGATTCATCG TATGATAATG GCCTTGGTGA GTGATTCAGC TTTATTCAAT
1441     ACCAGAGAAG GACGTGCTGG GAAGGTACAC AACTTCATGC TGGGCTTGAA TCTCAATACA
1501     TCTTATCCAC TGTCTCCTTT GAGTGACTTT GCCACACAGG ACTCCTTTGA TGATGATGAA
1561     CTGGATGCAG CTGTAGCAGA TCCTGATGAA TTTGAGCGAA TATATGAGCC TCTGGATGTC
1621     AAAAGTAAAA AGATTCATGT AGTGGACAGT GGGCTCACAT TTAACCTGCC GTATCCCTTG
1681     ATACTGAGAC CTCAGAGAGG GGTTGATCTC ATAATCTCCT TTGACTTTTC TGCAAGGCCA
1741     AGTGACTCTA GTCCTCCGTT CAAGGAACTT CTACTTGCAG AAAAGTGGGC TAAAATGAAC
1801     AAGCTCCCCT TTCCAAAGAT TGATCCTTAT GTGTTTGATC GGGAAGGGCT GAAGGAGTGC
1861     TATGTCTTTA AACCCAAGAA TCCTGATATG GAGAAAGATT GCCCAACCAT CATCCACTTT
1921     GTTCTGGCCA ACATCAACTT CAGAAAGTAC AGGGCTCCAG GTGTTCCAAG GGAAACTGAG
1981     GAAGAGAAAG AAATCGCTGA CTTTGATATT TTTGATGACC CAGAATCACC ATTTTCAACC
2041     TTCAATTTTC AATATCCAAA TCAAGCATTC AAAAGACTAC ATGATCTTAT GCACTTCAAT
2101     ACTCTGAACA ACATTGATGT GATAAAAGAA GCCATGGTTG AAAGCATTGA ATATAGAAGA
2161     CAGAATCCAT CTCGTTGCTC TGTTTCCCTT AGTAATGTTG AGGCAAGAAG ATTTTTCAAC
2221     AAGGAGTTTC TAAGTAAACC CAAAGCATAG

 

Quality Control:cDNA sequencing results is available upon request.