PLA2G1B Antibody (Antigen Affinity Purified)

PLA2G1B Antibody ( Antigen Affinity Purified ) Datasheet

PLA2G1B Antibody Product Information

• Immunogen: Recombinant Mouse PLA2G1B protein (Catalog#50329-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse PLA2G1B (rM PLA2G1B; Catalog#50329-M08H; NP_035237.1; Met 1-Cys146). PLA2G1B specific IgG was purified by Mouse PLA2G1B affinity chromatography.

PLA2G1B Antibody Usage Guide

• Specificity: Mouse PLA2G1B
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PLA2G1B in WB. Using a DAB detection system, the detection limit for Mouse PLA2G1B is approximately 0.5 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PLA2G1B. The detection limit for Mouse PLA2G1B is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PLA2G1B Antibody Background

Phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein which belongs to thephospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.

References

1. Labonte,E.D. et al., 2006, Diabetes. 55 (4): 935-41.
2. Mounier,C.M. et al.,2008, Br J Cancer. 98 (3): 587-95.
3. Hui,D.Y. et al., 2009,Br J Pharmacol. 157 (7): 1263-9.
4. Labonte,E.D. et al., 2010, FASEB J. 24 (7): 2516-24.