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Human PLA2G12B Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
HG13420-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PLA2G12B cDNA Clone Datasheet

 

PLA2G12B cDNA Clone Product Information

Gene Bank Ref.ID: NM_032562.2

cDNA Size:

585

Gene Synonym:

GXIIB; FKSG71; PLA2G13; GXIIIsPLA2; PLA2G12B

Source: Human

PLA2G12B cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens phospholipase A2, group XIIB DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 465-468 AGC deletion mutation.

PLA2G12B cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PLA2G12B cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PLA2G12B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCTGG CCAGTGGCTT CTTGGTTTTG TGGCTCAGCC TTGGGGGTGG CCTGGCTCAG
61       AGCGACACGA GCCCTGACAC GGAGGAGTCC TATTCAGACT GGGGCCTTCG GCACCTCCGG
121      GGAAGCTTTG AATCCGTCAA TAGCTACTTC GATTCTTTTC TGGAGCTGCT GGGAGGGAAG
181      AATGGAGTCT GTCAGTACAG GTGCCGATAT GGAAAGGCAC CAATGCCCAG ACCTGGCTAC
241      AAGCCCCAAG AGCCCAATGG CTGCGGCTCC TATTTCCTGG GTCTCAAGGT ACCAGAAAGT
301      ATGGACTTGG GCATTCCAGC AATGACAAAG TGCTGCAACC AGCTGGATGT CTGTTATGAC
361      ACTTGCGGTG CCAACAAATA TCGCTGTGAT GCAAAATTCC GATGGTGTCT CCACTCGATC
421      TGCTCTGACC TTAAGCGGAG TCTGGGCTTT GTCTCCAAAG TGGAAGCCTG TGATTCCCTG
481      GTTGACACTG TGTTCAACAC CGTGTGGACC TTGGGCTGCC GCCCCTTTAT GAATAGTCAG
541      CGGGCAGCTT GCATCTGTGC AGAGGAGGAG AAGGAAGAGT TATGA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

PLA2G12B related areas, pathways, and other information