PKCλ / PRKCI Protein (GST Tag)

Protein Kinase C Iota Type ( PKCλ / PRKCI ) Protein

PKCλ / PRKCI Protein Product Information

• Synonym: PKCI; DXS1179E; MGC26534; nPKC-iota; PRKCI
• Protein Construction: A DNA sequence encoding the human PKCλ ( NP_002731.4 ) ( Met 10 - Val 596 ) was fused with the GST tag at the C-terminus
• Source: Human
• Expression Host: Baculovirus-Insect cells

PKCλ / PRKCI Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PKCλ/GST chimera consists of 812 amino acids and has a predicted a molecular mass of 93.5 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5 mM PMSF, 10% glycerol, pH 7.4
• SDS-PAGE: PRKCI protein

PKCλ / PRKCI Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PKCλ / PRKCI Protein Description

Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCI contains one AGC-kinase C-terminal domain, one OPR domain, one phorbol-ester/DAG-type zinc finger and one protein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.

References

1. Selbie L.A. et al.,1993, J. Biol. Chem. 268:24296-302.
2. Diaz-Meco M.T. et al.,1996, Mol. Cell. Biol. 16:105-114.
3. Noda Y. et al.,2001, Genes Cells 6:107-119.
4. White W.O.et al.,2002, J. Cell. Biochem. 85:42-53.
5. Messerschmidt A. et al., 2005, J. Mol. Biol. 352:918-931.