All PKC iota reagents are produced in house and quality controlled, including 18 PKC iota Gene, 1 PKC iota Lysate, 1 PKC iota Protein, 2 PKC iota qPCR. All PKC iota reagents are ready to use.
Recombinant PKC iota proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-GST.
PKC iota cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each PKC iota of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCI contains one AGC-kinase C-terminal domain, one OPR domain, one phorbol-ester/DAG-type zinc finger and one protein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.
PKC iota References
Selbie L.A. et al.,1993, J. Biol. Chem. 268:24296-302.
Diaz-Meco M.T. et al.,1996, Mol. Cell. Biol. 16:105-114.
Noda Y. et al.,2001, Genes Cells 6:107-119.
White W.O.et al.,2002, J. Cell. Biochem. 85:42-53.
Messerschmidt A. et al., 2005, J. Mol. Biol. 352:918-931.