Mouse PRKCE Gene cDNA Clone / ORF Clone

PRKCE cDNA Clone Datasheet

PRKCE cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011104.3
• cDNA Size: 2214
• Gene Synonym: Pkce; PKC[e]; R75156; PKCepsilon; 5830406C15Rik; Prkce
• Source: Mouse

PRKCE cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protein kinase C, epsilon DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for seven point mutations 861 T/C, 918 T/C, 1089 T/C， 1176 C/T, 1236 G/A, 1554 A/G, 1578 C/T not causing the amino acid variation.

PRKCE cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PRKCE cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PRKCE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTAGTGT TCAATGGCCT TCTTAAGATC AAAATCTGCG AGGCGGTGAG CTTGAAGCCC
61       ACAGCCTGGT CGCTGCGCCA TGCGGTGGGA CCCCGGCCAC AGACGTTCCT TTTGGACCCC
121      TACATTGCCC TTAACGTGGA CGACTCGCGC ATCGGCCAAA CAGCCACCAA GCAAAAGACC
181      AACAGCCCGG CCTGGCACGA TGAGTTCGTC ACCGATGTGT GCAATGGGCG CAAGATCGAG
241      CTGGCTGTCT TTCACGACGC TCCTATCGGC TACGACGACT TCGTGGCCAA CTGCACCATC
301      CAGTTCGAGG AGCTGCTGCA GAATGGGAGC CGTCACTTCG AGGACTGGAT TGACCTGGAG
361      CCAGAAGGAA AAGTGTACGT GATCATCGAT CTCTCGGGAT CATCGGGTGA AGCCCCTAAA
421      GACAATGAAG AACGAGTGTT CAGGGAGCGT ATGCGGCCAA GGAAGCGGCA AGGGGCTGTC
481      AGGCGCAGGG TCCACCAGGT CAATGGCCAC AAGTTCATGG CCACCTACTT GCGGCAACCC
541      ACCTACTGCT CCCACTGCAG AGATTTCATC TGGGGTGTCA TAGGAAAACA GGGATATCAA
601      TGTCAAGTTT GCACTTGCGT TGTCCACAAG CGATGTCATG AGCTCATTAT TACAAAGTGC
661      GCTGGGCTGA AGAAACAGGA AACCCCTGAC GAGGTGGGCT CCCAACGGTT CAGCGTCAAC
721      ATGCCCCACA AGTTCGGGAT CCACAACTAC AAGGTCCCCA CGTTCTGTGA CCACTGTGGG
781      TCCCTGCTCT GGGGCCTCTT GCGGCAGGGC TTGCAGTGTA AAGTCTGCAA AATGAATGTT
841      CACCGGCGAT GTGAGACCAA CGTGGCTCCC AACTGTGGGG TAGACGCCAG AGGAATTGCC
901      AAAGTGCTGG CTGACCTCGG TGTTACTCCA GACAAAATCA CCAACAGTGG CCAAAGGAGG
961      AAAAAGCTCG CTGCTGGTGC TGAGTCCCCA CAGCCGGCTT CTGGAAACTC CCCATCTGAA
1021     GACGACCGAT CCAAGTCAGC GCCCACCTCC CCTTGTGACC AGGAACTAAA AGAACTTGAA
1081     AACAACATCC GGAAGGCCTT GTCATTTGAC AACCGAGGAG AGGAGCACCG AGCGTCGTCG
1141     GCCACCGATG GCCAGCTGGC AAGCCCCGGA GAGAATGGGG AAGTCCGGCC AGGCCAGGCC
1201     AAGCGCTTGG GGCTGGATGA GTTCAACTTC ATCAAAGTGT TGGGCAAAGG CAGCTTTGGC
1261     AAGGTCATGT TGGCGGAACT CAAAGGCAAA GATGAAGTCT ACGCTGTGAA GGTCTTGAAG
1321     AAGGACGTTA TCCTACAAGA CGATGATGTG GACTGCACAA TGACAGAGAA GAGGATTTTG
1381     GCTCTGGCTC GGAAACACCC TTATCTAACC CAACTCTATT GCTGCTTCCA GACCAAGGAC
1441     CGCCTCTTCT TCGTCATGGA ATATGTAAAT GGTGGAGACC TCATGTTCCA GATTCAGCGG
1501     TCCCGAAAAT TTGATGAGCC TCGTTCTCGG TTCTATGCCG CAGAGGTCAC ATCGGCCCTC
1561     ATGTTTCTCC ACCAGCATGG AGTGATCTAC AGGGATTTGA AACTGGACAA CATCCTTCTA
1621     GATGCAGAAG GCCACTGCAA GCTGGCTGAC TTTGGGATGT GCAAGGAAGG GATTATGAAT
1681     GGTGTGACAA CTACCACCTT CTGTGGGACT CCTGACTACA TAGCTCCAGA GATCCTACAG
1741     GAGTTGGAGT ACGGCCCCTC AGTGGACTGG TGGGCCCTGG GGGTGCTGAT GTACGAGATG
1801     ATGGCTGGGC AGCCCCCCTT TGAAGCTGAC AACGAGGACG ACTTGTTCGA ATCCATCCTT
1861     CATGATGATG TTCTCTATCC TGTCTGGCTT AGCAAGGAAG CTGTCAGCAT CCTGAAAGCT
1921     TTCATGACCA AGAACCCGCA CAAGCGCCTG GGCTGTGTGG CAGCGCAGAA CGGGGAGGAC
1981     GCCATCAAGC AACATCCATT CTTCAAGGAG ATTGACTGGG TACTGCTGGA GCAGAAGAAA
2041     ATCAAGCCCC CCTTCAAGCC GAGAATTAAA ACCAAAAGAG ATGTCAATAA CTTTGACCAA
2101     GACTTTACGC GGGAAGAGCC AATACTTACA CTTGTGGATG AAGCAATCAT TAAGCAGATC
2161     AACCAGGAAG AATTCAAAGG CTTCTCCTAC TTTGGTGAAG ACCTGATGCC CTGA

 

Quality Control:cDNA sequencing results is available upon request.