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Human PRKCD Gene ORF cDNA clone in cloning vector

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    Human PRKCD cDNA Clone Product Information
    NCBI RefSeq:NM_006254.3
    RefSeq ORF Size:2031bp
    cDNA Description:Full length Clone DNA of Homo sapiens protein kinase C, delta.
    Gene Synonym:MAY1, PKCD, MGC49908, nPKC-delta
    Species:Human
    Vector:pMD18-T Simple Vector
    Plasmid:pMD-PRKCD
    Restriction Site:
    Tag Sequence:
    Sequence Description:Identical with the Gene Bank Ref. ID sequence except for three point mutations: 1441 C/T ,1782 C/G and 1857 T/C not causing the amino acid variation.
    Sequencing primers:M13-47 and RV-M
    ( We provide with PRKCD qPCR primers for gene expression analysis, HP100905 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
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    References
  • Cross T, et al. (2000) PKC-delta is an apoptotic lamin kinase. Oncogene. 19(19): 2331-7.
  • Song JS, et al. (1998) Tyrosine phosphorylation-dependent and -independent associations of protein kinase C-delta with Src family kinases in the RBL-2H3 mast cell line: regulation of Src family kinase activity by protein kinase C-delta. Oncogene. 16(26): 3357-68.
  • Shanmugam M, et al. (1998) Association of PKC delta and active Src in PMA-treated MCF-7 human breast cancer cells. Oncogene. 16(13): 1649-54.
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    Catalog: HG10769-M
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